Toxoplasma gondii infection in European brown hares (Lepus europaeus) in Brazil

• Published in: Parasitology research (1987) Vol. 123; no. 5; p. 222
• Main Authors: Arias-Pacheco, Carmen, Perin, Patricia Parreira, de Oliveira Andrade, Lívia, Gomes, Jonathan Silvestre, Oliveira, Wilson Junior, Mendonça, Talita Oliveira, de Oliveira Zolla, Natália, Cavallieri, Ana Carolina, da Silva, Douglas Aparecido, Castilho, Pablo Menegon, de Barros, Luiz Daniel, Garcia, João Luis, Lux Hoppe, Estevam G.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 27-05-2024; Springer Nature B.V
• Subjects: Animals; Bioassays; Biological Assay; Biomedical and Life Sciences; Biomedicine; Brazil - epidemiology; Carnivores; Domestic animals; Female; Hares - parasitology; Immunology; Infections; Male; Medical Microbiology; Mice; Microbiology; Prevalence; Serology; Small intestine; Toxoplasma - isolation & purification; Toxoplasma gondii; Toxoplasmosis; Toxoplasmosis, Animal - diagnosis; Toxoplasmosis, Animal - epidemiology; Toxoplasmosis, Animal - parasitology; Brazil; Rio Grande do Sul Brazil; Toxoplasmosis; Invasive alien species; Zoonosis; Lepus europaeus
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-024-08248-4

Toxoplasmosis is a worldwide zoonosis that affects warm-blooded animals, including humans. Wild animals can act as intermediate hosts of this pathogen; thus, this study aims to detect
Toxoplasma gondii
infection in invasive European brown hares in Brazil. For this, 72 wild European brown hares were captured from July 2020 to June 2022 in three Brazilian states: São Paulo, Paraná, and Rio Grande do Sul. The diagnostic of
Toxoplasma gondii
infection was performed by bioassay in mouse, histopathology in Hematoxylin-Eosin-stained tissue sections (brain, liver, lungs, kidneys, and small intestine), serology by IFAT, and molecular techniques by conventional PCR and qPCR. The combined prevalence of the different diagnostic methods was 51.4% (37/72, CI= 40.1 - 62.6 %), and there was no statistical difference between sexes, age range, or geographical region of the hosts. Mouse bioassay was the technique that detected more positive hares. To our knowledge, this is the first confirmation of
Toxoplasma gondii
infection in invasive European brown hares in Brazil. These animals act as reservoirs and potential infection source for carnivores and other wild and domestic animals, including humans, thus contributing to perpetuate the disease cycle in São Paulo, Paraná, and Rio Grande do Sul States. Research such as the present study is necessary to raise awareness about the role of animals in the disease cycle.