TiO2-PANI based anti-typhi immobilized nanosensor for salmonella typhi detection
TiO2-PANI electrode is employed to develop the nano biosensor for diagnosis the Typhoid with high accuracy. Sensor has prepared by spin coating method on a silicon substrate by depositing TiO2-PANI as a sensing material. The nano particle was characterised by using X-Ray diffraction, UV- visible spe...
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| Published in: | Materials today : proceedings Vol. 3; no. 6; pp. 1772 - 1777 |
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2016
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| Abstract | TiO2-PANI electrode is employed to develop the nano biosensor for diagnosis the Typhoid with high accuracy. Sensor has prepared by spin coating method on a silicon substrate by depositing TiO2-PANI as a sensing material. The nano particle was characterised by using X-Ray diffraction, UV- visible spectrometry to know the structural and optical properties. X-Ray diffraction pattern shows the peak in between 20-30o. The result of UV- visible spectroscopy reviles the absorption curve at 390 nm. The morphology of TiO2 nanostructure is confirmed by FESEM. The Antibody (Anti-typhi) immobilized on TiO2-PANI have a sensitive detection of salmonella antigens from the sample. The linear variation of resistance indicates the detection of salmonella typhi in earlier stage with accuracy to the different concentration of salmonella typhi concentration. |
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| AbstractList | TiO2-PANI electrode is employed to develop the nano biosensor for diagnosis the Typhoid with high accuracy. Sensor has prepared by spin coating method on a silicon substrate by depositing TiO2-PANI as a sensing material. The nano particle was characterised by using X-Ray diffraction, UV- visible spectrometry to know the structural and optical properties. X-Ray diffraction pattern shows the peak in between 20-30o. The result of UV- visible spectroscopy reviles the absorption curve at 390 nm. The morphology of TiO2 nanostructure is confirmed by FESEM. The Antibody (Anti-typhi) immobilized on TiO2-PANI have a sensitive detection of salmonella antigens from the sample. The linear variation of resistance indicates the detection of salmonella typhi in earlier stage with accuracy to the different concentration of salmonella typhi concentration. |
| Author | Krishna, Vijaya Sanjay, P.N. Jagadeesh Prasad, D. K. Naveen Kumar, S. Gayithri, K.C. |
| Author_xml | – sequence: 1 givenname: P.N. surname: Sanjay fullname: Sanjay, P.N. email: sanjay.paramale@gmail.com organization: Department of Electronics, Mangalore University, Mangalore, India – sequence: 2 givenname: K.C. surname: Gayithri fullname: Gayithri, K.C. organization: Department of Electronics, Mangalore University, Mangalore, India – sequence: 3 givenname: S. surname: K. Naveen Kumar fullname: K. Naveen Kumar, S. organization: Department of Electronics, Mangalore University, Mangalore, India – sequence: 4 givenname: Vijaya surname: Krishna fullname: Krishna, Vijaya organization: Department of Electronics, Mangalore University, Mangalore, India – sequence: 5 givenname: D. surname: Jagadeesh Prasad fullname: Jagadeesh Prasad, D. organization: Department of Chemistry, Mangalore University, Mangalore, India |
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| Cites_doi | 10.1039/C3TA14173J 10.1016/0035-9203(89)90407-0 10.1109/ICANMEET.2013.6609325 10.4269/ajtmh.1986.35.836 10.1086/344953 10.4269/ajtmh.2004.70.323 10.1038/srep06891 10.1136/pmj.76.892.80 10.1093/infdis/153.2.358 10.1016/S1567-1348(02)00090-4 10.1128/JCM.43.1.431-432.2005 10.1016/j.aca.2006.03.078 10.3390/s90201033 10.1039/b414248a 10.4269/ajtmh.1978.27.795 10.1002/adma.201103334 |
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Clin. Microbiol doi: 10.1128/JCM.43.1.431-432.2005 contributor: fullname: Prakash – volume: 568 start-page: 259 year: 2006 ident: 10.1016/j.matpr.2016.04.073_b0110 article-title: Recent developments in bio-molecular-electronics technique for food pathogens publication-title: AnalyticaChemica Acta doi: 10.1016/j.aca.2006.03.078 contributor: fullname: Arora – ident: 10.1016/j.matpr.2016.04.073_b0055 – volume: 9 start-page: 1033 year: 2009 ident: 10.1016/j.matpr.2016.04.073_b0120 article-title: Recent advances in nanotechnology applied to biosensors publication-title: Sensors doi: 10.3390/s90201033 contributor: fullname: Zhang – volume: 130 start-page: 421 year: 2005 ident: 10.1016/j.matpr.2016.04.073_b0125 article-title: Nanomaterial-based electrochemical biosensors publication-title: Analyst doi: 10.1039/b414248a contributor: fullname: Wang – ident: 10.1016/j.matpr.2016.04.073_b0070 doi: 10.4269/ajtmh.1978.27.795 – start-page: 213 year: 1980 ident: 10.1016/j.matpr.2016.04.073_b0050 article-title: Rapid method for detection, identification, susceptibility testing of enteric pathogens publication-title: Journal of clinical microbiology contributor: fullname: Rockill – volume: 30 start-page: 2513 year: 1992 ident: 10.1016/j.matpr.2016.04.073_b0085 article-title: Nguan, P publication-title: Echeverria, Diagnosis of tyhoid fever by detection of salmonella typhi antigen in urine, J ClinMicrobiol contributor: fullname: Chaicumpa – volume: 24 start-page: 34 year: 2012 ident: 10.1016/j.matpr.2016.04.073_b0115 article-title: Organic thinfilm transistors for chemical and biological sensing publication-title: Advanced materials doi: 10.1002/adma.201103334 contributor: fullname: Lin |
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