Combination effect of azadirachtin and chlorantraniliprole with three synergists against a serious invasive agricultural pest Spodoptera frugiperda (Lepidoptera: Noctuidae)

• Published in: Biocatalysis and agricultural biotechnology Vol. 55; p. 102992
• Main Authors: Muthusamy, Ranganathan, Vengateswari, Govindaraj, Kumarasamy, Suresh, Pandi, Ramkumar, Prasannakumar, N.R., Arul, Dhayalan, Dhanapal, Rajendran, Kariyanna, B., Karthik, Chinnannan, Malathi, Govindhan, Ramkumar, Govindaraju
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-01-2024
• Subjects: Azadirachtin; Detoxification enzymes; Resistance management; Synergist; Detoxification enzymes; Azadirachtin; Synergist; Resistance management
• ISSN: 1878-8181; 1878-8181
• DOI: 10.1016/j.bcab.2023.102992

The fall armyworm, Spodoptera frugiperda, is a newly invading polyphagous pest that affects crops such as maize, sorghum, and corn in India. The pest has developed resistance to various chemical insecticides, including Bt toxins and insect growth regulators. Therefore, there is an urgent need for alternative methods to manage this pest. The present study aimed to investigate the effect of three synergists in combination with pesticides. The results showed a high resistance ratio to chlorantraniliprole (RR-52.7) and low resistance to azadirachtin (RR-3.8) in the HSR field population compared to the susceptible population (Sus). In vitro enzyme inhibition experiments with PBO and TPP synergists revealed that resistance to chlorantraniliprole was mediated by esterase, GST and MFO. However less resistance to azadirachtin was mediated by esterase enzyme. The field population exhibited a 2.0-fold higher esterase activity (20 μmol/min/mg protein) in response to chlorantraniliprole, followed by MFO activity (30–32 μmol/min/mg protein). Gene expression analysis revealed four genes (CYP321A7, CYP321B1, CYP4C71, and CYP4G74) were differentially expressed in the HSR population (4.0–6.0-fold) in chlorantraniliprole treatment and no expressions were seen in azadirachtin treatment. Overall the study concludes that, possible mechanism of chlorantraniliprole resistance in S. frugiperda mediated by P450s, esterases and GST and less resistance to azadirachtin by esterase suggesting that combination of synergists with azadirachtin could be useful for management of Spodoptera frugiperda.
•Chlorantraniliprole LC50 value increased in HSR field population 52.7 ppm then susceptible S. frugiperda.•High synergism ratio was found with PBO (6.44) and TPP (4.71) in HSR population.•MFO, GST and esterase activity was inhibited in in-vitro study with PBO synergist.•Less resistance to azadirachtin was found with and without synergist combination.•Cytochrome genes CYP321A, CYP321B1, CYP4C71 and CYP4G74 overexpressed in and down regulated by PBO.