Expression Analysis of Circulating microRNAs in Saliva and Plasma for the Identification of Clinically Relevant Biomarkers for Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders

This study aims to evaluate the expression of salivary and plasmatic miRNAs as diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs). A total of 25 patients were divided into three groups, according to their diagnosis: OSCC patien...

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Published in:	Cancers Vol. 16; no. 17; p. 2990
Main Authors:	Rocchetti, Federica, Tenore, Gianluca, Macali, Federica, Vicidomini, Teresa, Podda, Gian Marco, Fantozzi, Paolo Junior, Silvestri, Valentina, Porzio, Virginia, Valentini, Virginia, Ottini, Laura, Richetta, Antonio Giovanni, Valentini, Valentino, Della Monaca, Marco, Grenga, Camilla, Polimeni, Antonella, Romeo, Umberto
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 28-08-2024
Subjects:	Alcohol Biomarkers Biopsy Body fluids Diagnosis Disease management Ethics Health care Medical prognosis MicroRNAs miRNA Oral cancer Oral carcinoma Oral squamous cell carcinoma Patients Plasma Saliva Squamous cell carcinoma Statistical analysis Tobacco Variance analysis Canada United States > US microRNAs oral potentially malignant disorders oral squamous cell carcinoma liquid biopsy non-invasive biomarkers
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Abstract	This study aims to evaluate the expression of salivary and plasmatic miRNAs as diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs). A total of 25 patients were divided into three groups, according to their diagnosis: OSCC patients ( = 14); OPMDs patients ( = 6); and healthy controls ( = 5). At the time at diagnosis/enrolment, patients underwent salivary and plasmatic collection. The expression of miRNA -21, -31, -138, -145, -184, and -424 were evaluated by real-time PCR. An F-test and ANOVA test were performed to evaluate the miRNA levels (significance at < 0.05). By comparing miRNA expression levels from saliva, a statistically significant difference emerged in the expression of miR-138 and miR-424 between the three groups ( < 0.05). In particular, these two miRNAs showed decreased expression levels in saliva samples from OSCC and OPMD patients compared to those from healthy controls. On the other hand, miRNA expression levels in plasma were low in all the groups, and no statistically significant differences were found. Overall, our results showed that liquid biopsy from saliva may be a useful tool for the identification of diagnostic molecular biomarkers in OSCC and OPMDs.
AbstractList	Simple SummaryIn recent years, liquid biopsy has been introduced as a new method for the detection and management of cancer, but the studies on oral squamous cell carcinoma (OSCC) are lacking conclusive evidence. The aim of this study was to evaluate the expression of six circulating salivary and plasmatic miRNAs (-21, -31, -138, -145, -184, and -424) as diagnostic biomarkers in patients affected by OSCC and by oral potentially malignant disorders (OPMDs). Our results showed that liquid biopsy from saliva may be a useful tool for the identification of these biomarkers; in particular, miR-138 and miR-424 showed decreased expression levels in saliva samples in OSCC and OPMD patients compared to healthy controls. The introduction of liquid biopsy in daily clinical practice could revolutionize the approach to oral lesions by allowing the mass screening, stratification and monitoring of patients at risk, the monitoring of the response to treatment and the early identification of any recurrences.AbstractThis study aims to evaluate the expression of salivary and plasmatic miRNAs as diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs). A total of 25 patients were divided into three groups, according to their diagnosis: OSCC patients (n = 14); OPMDs patients (n = 6); and healthy controls (n = 5). At the time at diagnosis/enrolment, patients underwent salivary and plasmatic collection. The expression of miRNA -21, -31, -138, -145, -184, and -424 were evaluated by real-time PCR. An F-test and ANOVA test were performed to evaluate the miRNA levels (significance at p < 0.05). By comparing miRNA expression levels from saliva, a statistically significant difference emerged in the expression of miR-138 and miR-424 between the three groups (p < 0.05). In particular, these two miRNAs showed decreased expression levels in saliva samples from OSCC and OPMD patients compared to those from healthy controls. On the other hand, miRNA expression levels in plasma were low in all the groups, and no statistically significant differences were found. Overall, our results showed that liquid biopsy from saliva may be a useful tool for the identification of diagnostic molecular biomarkers in OSCC and OPMDs. This study aims to evaluate the expression of salivary and plasmatic miRNAs as diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs). A total of 25 patients were divided into three groups, according to their diagnosis: OSCC patients (n = 14); OPMDs patients (n = 6); and healthy controls (n = 5). At the time at diagnosis/enrolment, patients underwent salivary and plasmatic collection. The expression of miRNA -21, -31, -138, -145, -184, and -424 were evaluated by real-time PCR. An F-test and ANOVA test were performed to evaluate the miRNA levels (significance at p < 0.05). By comparing miRNA expression levels from saliva, a statistically significant difference emerged in the expression of miR-138 and miR-424 between the three groups (p < 0.05). In particular, these two miRNAs showed decreased expression levels in saliva samples from OSCC and OPMD patients compared to those from healthy controls. On the other hand, miRNA expression levels in plasma were low in all the groups, and no statistically significant differences were found. Overall, our results showed that liquid biopsy from saliva may be a useful tool for the identification of diagnostic molecular biomarkers in OSCC and OPMDs.This study aims to evaluate the expression of salivary and plasmatic miRNAs as diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs). A total of 25 patients were divided into three groups, according to their diagnosis: OSCC patients (n = 14); OPMDs patients (n = 6); and healthy controls (n = 5). At the time at diagnosis/enrolment, patients underwent salivary and plasmatic collection. The expression of miRNA -21, -31, -138, -145, -184, and -424 were evaluated by real-time PCR. An F-test and ANOVA test were performed to evaluate the miRNA levels (significance at p < 0.05). By comparing miRNA expression levels from saliva, a statistically significant difference emerged in the expression of miR-138 and miR-424 between the three groups (p < 0.05). In particular, these two miRNAs showed decreased expression levels in saliva samples from OSCC and OPMD patients compared to those from healthy controls. On the other hand, miRNA expression levels in plasma were low in all the groups, and no statistically significant differences were found. Overall, our results showed that liquid biopsy from saliva may be a useful tool for the identification of diagnostic molecular biomarkers in OSCC and OPMDs. This study aims to evaluate the expression of salivary and plasmatic miRNAs as diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs). A total of 25 patients were divided into three groups, according to their diagnosis: OSCC patients ( = 14); OPMDs patients ( = 6); and healthy controls ( = 5). At the time at diagnosis/enrolment, patients underwent salivary and plasmatic collection. The expression of miRNA -21, -31, -138, -145, -184, and -424 were evaluated by real-time PCR. An F-test and ANOVA test were performed to evaluate the miRNA levels (significance at < 0.05). By comparing miRNA expression levels from saliva, a statistically significant difference emerged in the expression of miR-138 and miR-424 between the three groups ( < 0.05). In particular, these two miRNAs showed decreased expression levels in saliva samples from OSCC and OPMD patients compared to those from healthy controls. On the other hand, miRNA expression levels in plasma were low in all the groups, and no statistically significant differences were found. Overall, our results showed that liquid biopsy from saliva may be a useful tool for the identification of diagnostic molecular biomarkers in OSCC and OPMDs. This study aims to evaluate the expression of salivary and plasmatic miRNAs as diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs). A total of 25 patients were divided into three groups, according to their diagnosis: OSCC patients (n = 14); OPMDs patients (n = 6); and healthy controls (n = 5). At the time at diagnosis/enrolment, patients underwent salivary and plasmatic collection. The expression of miRNA -21, -31, -138, -145, -184, and -424 were evaluated by real-time PCR. An F-test and ANOVA test were performed to evaluate the miRNA levels (significance at p < 0.05). By comparing miRNA expression levels from saliva, a statistically significant difference emerged in the expression of miR-138 and miR-424 between the three groups (p < 0.05). In particular, these two miRNAs showed decreased expression levels in saliva samples from OSCC and OPMD patients compared to those from healthy controls. On the other hand, miRNA expression levels in plasma were low in all the groups, and no statistically significant differences were found. Overall, our results showed that liquid biopsy from saliva may be a useful tool for the identification of diagnostic molecular biomarkers in OSCC and OPMDs.
Author	Rocchetti, Federica Richetta, Antonio Giovanni Fantozzi, Paolo Junior Podda, Gian Marco Della Monaca, Marco Vicidomini, Teresa Silvestri, Valentina Valentini, Valentino Macali, Federica Polimeni, Antonella Valentini, Virginia Tenore, Gianluca Grenga, Camilla Romeo, Umberto Ottini, Laura Porzio, Virginia
Author_xml	– sequence: 1 givenname: Federica surname: Rocchetti fullname: Rocchetti, Federica organization: Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy – sequence: 2 givenname: Gianluca orcidid: 0000-0001-9963-8052 surname: Tenore fullname: Tenore, Gianluca organization: Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy – sequence: 3 givenname: Federica surname: Macali fullname: Macali, Federica organization: Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy – sequence: 4 givenname: Teresa surname: Vicidomini fullname: Vicidomini, Teresa organization: Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy – sequence: 5 givenname: Gian Marco orcidid: 0000-0002-4981-5396 surname: Podda fullname: Podda, Gian Marco organization: Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy – sequence: 6 givenname: Paolo Junior orcidid: 0000-0002-6807-3391 surname: Fantozzi fullname: Fantozzi, Paolo Junior organization: Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy – sequence: 7 givenname: Valentina orcidid: 0000-0003-0712-9379 surname: Silvestri fullname: Silvestri, Valentina organization: Department of Molecular Medicine, Sapienza University of Rome, 00161 Rome, Italy – sequence: 8 givenname: Virginia surname: Porzio fullname: Porzio, Virginia organization: Department of Molecular Medicine, Sapienza University of Rome, 00161 Rome, Italy – sequence: 9 givenname: Virginia orcidid: 0000-0003-3393-7185 surname: Valentini fullname: Valentini, Virginia organization: Department of Molecular Medicine, Sapienza University of Rome, 00161 Rome, Italy – sequence: 10 givenname: Laura orcidid: 0000-0001-8030-0449 surname: Ottini fullname: Ottini, Laura organization: Department of Molecular Medicine, Sapienza University of Rome, 00161 Rome, Italy – sequence: 11 givenname: Antonio Giovanni orcidid: 0000-0002-8755-9178 surname: Richetta fullname: Richetta, Antonio Giovanni organization: Department of Internal Medicine and Medical Specialties, Sapienza University of Rome, 00161 Rome, Italy – sequence: 12 givenname: Valentino surname: Valentini fullname: Valentini, Valentino organization: Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy – sequence: 13 givenname: Marco orcidid: 0000-0001-6235-9754 surname: Della Monaca fullname: Della Monaca, Marco organization: Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy – sequence: 14 givenname: Camilla orcidid: 0000-0001-7822-5953 surname: Grenga fullname: Grenga, Camilla organization: Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy – sequence: 15 givenname: Antonella orcidid: 0000-0002-2679-7607 surname: Polimeni fullname: Polimeni, Antonella organization: Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy – sequence: 16 givenname: Umberto orcidid: 0000-0003-2439-2187 surname: Romeo fullname: Romeo, Umberto organization: Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy
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Snippet	This study aims to evaluate the expression of salivary and plasmatic miRNAs as diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) and... Simple SummaryIn recent years, liquid biopsy has been introduced as a new method for the detection and management of cancer, but the studies on oral squamous...
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SubjectTerms	Alcohol Biomarkers Biopsy Body fluids Diagnosis Disease management Ethics Health care Medical prognosis MicroRNAs miRNA Oral cancer Oral carcinoma Oral squamous cell carcinoma Patients Plasma Saliva Squamous cell carcinoma Statistical analysis Tobacco Variance analysis
Title	Expression Analysis of Circulating microRNAs in Saliva and Plasma for the Identification of Clinically Relevant Biomarkers for Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders
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