Bacterial pathogens from Diprion pini L. (Hymenoptera: Diprionidae) and their biocontrol potential

• Published in: Biológia Vol. 77; no. 10; pp. 3001 - 3013
• Main Authors: Çelik, Tayyib, Sevim, Ali
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-10-2022; Springer Nature B.V
• Subjects: Arthrobacter; Bacteria; Biological control; Biomedical and Life Sciences; Cell Biology; Coniferous forests; Diprion pini; Diprionidae; Enterobacter; Forest soils; Gene sequencing; Hymenoptera; Insecticides; Insects; Klebsiella; Larvae; Life Sciences; Microbiology; Microorganisms; Mortality; Original Article; Pest control; Pests; Phylogeny; Plant Sciences; rRNA; Strains (organisms); Toxins; Zoology; Bacteria; The common pine sawfly; Virulence; Biological control
• ISSN: 1336-9563; 0006-3088; 1336-9563
• DOI: 10.1007/s11756-022-01161-0

Microbial insecticides have become increasingly important in recent years for the control of insect pests. Among microbial insecticides, insect pathogenic bacteria and their toxins have been the most commercially successful and hold potential for further development. In this study, we investigated the isolation and identification of some potential pathogenic bacteria from
Diprion pini
L. (Hymenoptera: Diprionidae) which is a serious pest of pine forests worldwide. A total of eighteen bacteria were isolated, ten bacteria from dead
D. pini
larvae and eight bacteria from healthy larvae. The bacterial strains were characterized by their morphological features, 16 S rRNA gene sequencing and phylogenetic analysis. In addition, these bacteria and four
Bacillus thuringiensis
strains (isolated from pine forest soil) were tested against
D. pini
larvae under laboratory conditions. The bacterial isolates were identified as
Kluyvera intermedia
O-1, O-8, O-10 and S-3,
Proteus mirabilis
O-2,
Klebsiella oxytoca
O-3,
Bacillus
sp. O-4,
Pantoea agglomerans
O-5 and S-5,
Serratia marcescens
O-6,
Pseudomonas
sp. O-7,
Acinetobacter
sp. O-9,
Enterobacter
sp. S-1 and S-9,
Bacillus pumilus
S-2,
Enterobacter cancerogenus
S-4,
Pseudoclavibacter
sp. S-6 and
Arthrobacter
sp. S-8. All isolates showed different insecticidal activity against the pest and the highest mortality was obtained from
P. mirabilis
O-2 with 100% within ten days after exposure. The highest mortality among
B. thuringiensis
strains was obtained from
B. thuringiensis
37 − 4 with 56.67%. This is the first study of determination of the culturable bacterial diversity within
D. pini
and the obtained results might be beneficial for biocontrol of
D. pini
.