Optimization of Electroporation Conditions for Bacillus pumilus 3–19 Strain

Electroporation is the process of using electrical impulses to create temporary pores in the plasma membrane which, in turn, enables the penetration of nucleic acids into the cytoplasm of a cell. This method is widely used for the rapid and efficient introduction of foreign DNA into a wide range of...

Full description

Saved in:

Bibliographic Details
Published in:	BioNanoScience Vol. 12; no. 3; pp. 752 - 756
Main Authors:	Danilova, I. V., Rudakova, N. L., Vasilyeva, Y. A., Gilmutdinova, A. I., Diadkina, I. V., Khasanov, D. I., Sharipova, M. R.
Format:	Journal Article
Language:	English
Published:	New York Springer US 01-09-2022 Springer Nature B.V
Subjects:	Bacillus pumilus Biological and Medical Physics Biomaterials Biophysics Buffers Cell culture Cell viability Circuits and Systems Cytoplasm Deoxyribonucleic acid DNA Efficiency Electric field strength Electric pulses Electroporation Engineering Nanotechnology Nucleic acids Optimization Electroporation Transformation Competent cells Bacterial growth phase Electric field strength
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Abstract	Electroporation is the process of using electrical impulses to create temporary pores in the plasma membrane which, in turn, enables the penetration of nucleic acids into the cytoplasm of a cell. This method is widely used for the rapid and efficient introduction of foreign DNA into a wide range of cells. Cell viability and electrotransfection efficiency depend on various experimental factors, including the impulse form, vector concentration, cell type (density), properties of the electroporation buffer, and the growth phase of the bacterial culture. In this work, we investigated the optimal conditions for the transformation of the Bacillus pumilus 3–19 strain using electroporation. Competent B. pumilus cells were obtained on the 4th hour of culture growth using PEB1 electroporation buffer and SOC1 (Super Optimal broth with Catabolic repressor) medium, while the electric field strength was 12 kV/cm. With these parameters, the transformation efficiency of bacillus cells was 56.3 transformants/μg DNA. Thus, the rational choice of pulsation conditions and buffering compositions is critical for the design of electroporation protocols to maximize the viability and efficiency of electrotransfection.
AbstractList	Electroporation is the process of using electrical impulses to create temporary pores in the plasma membrane which, in turn, enables the penetration of nucleic acids into the cytoplasm of a cell. This method is widely used for the rapid and efficient introduction of foreign DNA into a wide range of cells. Cell viability and electrotransfection efficiency depend on various experimental factors, including the impulse form, vector concentration, cell type (density), properties of the electroporation buffer, and the growth phase of the bacterial culture. In this work, we investigated the optimal conditions for the transformation of the Bacillus pumilus 3–19 strain using electroporation. Competent B. pumilus cells were obtained on the 4th hour of culture growth using PEB1 electroporation buffer and SOC1 (Super Optimal broth with Catabolic repressor) medium, while the electric field strength was 12 kV/cm. With these parameters, the transformation efficiency of bacillus cells was 56.3 transformants/μg DNA. Thus, the rational choice of pulsation conditions and buffering compositions is critical for the design of electroporation protocols to maximize the viability and efficiency of electrotransfection. Electroporation is the process of using electrical impulses to create temporary pores in the plasma membrane which, in turn, enables the penetration of nucleic acids into the cytoplasm of a cell. This method is widely used for the rapid and efficient introduction of foreign DNA into a wide range of cells. Cell viability and electrotransfection efficiency depend on various experimental factors, including the impulse form, vector concentration, cell type (density), properties of the electroporation buffer, and the growth phase of the bacterial culture. In this work, we investigated the optimal conditions for the transformation of the Bacillus pumilus 3–19 strain using electroporation. Competent B. pumilus cells were obtained on the 4th hour of culture growth using PEB1 electroporation buffer and SOC1 (Super Optimal broth with Catabolic repressor) medium, while the electric field strength was 12 kV/cm. With these parameters, the transformation efficiency of bacillus cells was 56.3 transformants/μg DNA. Thus, the rational choice of pulsation conditions and buffering compositions is critical for the design of electroporation protocols to maximize the viability and efficiency of electrotransfection.
Author	Rudakova, N. L. Khasanov, D. I. Diadkina, I. V. Danilova, I. V. Sharipova, M. R. Vasilyeva, Y. A. Gilmutdinova, A. I.
Author_xml	– sequence: 1 givenname: I. V. orcidid: 0000-0002-2437-8468 surname: Danilova fullname: Danilova, I. V. email: Danilova146@mail.ru organization: Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University – sequence: 2 givenname: N. L. surname: Rudakova fullname: Rudakova, N. L. organization: Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University – sequence: 3 givenname: Y. A. surname: Vasilyeva fullname: Vasilyeva, Y. A. organization: Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University – sequence: 4 givenname: A. I. surname: Gilmutdinova fullname: Gilmutdinova, A. I. organization: Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University – sequence: 5 givenname: I. V. surname: Diadkina fullname: Diadkina, I. V. organization: Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University – sequence: 6 givenname: D. I. surname: Khasanov fullname: Khasanov, D. I. organization: Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University – sequence: 7 givenname: M. R. surname: Sharipova fullname: Sharipova, M. R. organization: Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University
BookMark	eNp9kLtOAzEQRS0UJELID1CtRL0wtvdhlxCFhxSUAqgtxw_kaGMv9m4BFf_AH_IlbFgEHdPc0ejeO9I5RhMfvEHoFMM5BqgvEiZVxXIgJAfgjOTkAE0J5jjHVcEnvzuFIzRPaQvD1FBRRqfoft12bufeZOeCz4LNlo1RXQxtiONpEbx2-y1lNsTsSirXNH3K2n7n9ko_3z8wzx66KJ0_QYdWNsnMf3SGnq6Xj4vbfLW-uVtcrnJFCt7lG7CEGV1ixSzThkm-4VaVuCwKA5JWNTZYG4l5aYBgoEQrybU1vCK6VsDoDJ2NvW0ML71JndiGPvrhpSA1AC0p5TC4yOhSMaQUjRVtdDsZXwUGsScnRnJiICe-yQkyhOgYSoPZP5v4V_1P6guA1XOI
CitedBy_id	crossref_primary_10_3390_microorganisms11061508
Cites_doi	10.3389/fmicb.2015.01431 10.1038/s41598-020-66792-2 10.1111/1751-7915.12771 10.1099/00221287-136-2-255 10.2323/jgam.61.124 10.1186/s12896-018-0451-0 10.1007/s00253-012-3987-2 10.1186/s13567-019-0696-1 10.1007/s12223-013-0223-0 10.31857/S0026898420010176 10.1155/2017/8525912 10.1007/s42770-020-00241-0 10.1007/s00253-020-10852-0 10.1002/mbo3.663 10.1016/j.cub.2016.11.023 10.3390/ani10091604 10.3791/50684 10.1007/s00253-009-2349-1 10.3389/fmicb.2016.01195 10.1093/aobpla/plz036 10.3390/md16090326 10.1007/s10068-019-00691-9 10.3389/fmicb.2019.02548 10.1128/iai.32.3.1295-1297.1981 10.1007/s11033-009-9704-2 10.1371/journal.pone.0217255 10.1128/aem.55.3.604-610.1989 10.1093/nar/16.13.6127
ContentType	Journal Article
Copyright	The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022 The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022.
Copyright_xml	– notice: The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022 – notice: The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022.
DBID	AAYXX CITATION
DOI	10.1007/s12668-022-00982-2
DatabaseName	CrossRef
DatabaseTitle	CrossRef
DatabaseTitleList
DeliveryMethod	fulltext_linktorsrc
Discipline	Engineering
EISSN	2191-1649
EndPage	756
ExternalDocumentID	10_1007_s12668_022_00982_2
GrantInformation_xml	– fundername: Российский Фонд Фундаментальных Исследований (РФФИ) grantid: 19-08-00853 funderid: http://dx.doi.org/10.13039/501100002261
GroupedDBID	-58 -5G -BR -EM -~C 0R~ 0VY 203 29~ 30V 4.4 406 408 409 96X AAAVM AAFGU AAHNG AAIAL AAJKR AANZL AAPBV AARHV AARTL AATNV AATVU AAUYE AAWCG AAYFA AAYIU AAYQN AAYTO AAZMS ABBXA ABDZT ABECU ABFGW ABFTV ABJNI ABJOX ABKAS ABKCH ABMQK ABQBU ABSXP ABTEG ABTHY ABTKH ABTMW ABULA ABXPI ACBMV ACBRV ACBYP ACGFS ACHSB ACIGE ACIPQ ACIWK ACKNC ACMLO ACOKC ACPRK ACREN ACTTH ACVWB ACWMK ADHHG ADHIR ADINQ ADKNI ADKPE ADMDM ADOXG ADRFC ADTPH ADURQ ADYFF ADYOE ADZKW AEBTG AEFTE AEGNC AEJHL AEJRE AEOHA AEPYU AESKC AESTI AETCA AEVLU AEVTX AEXYK AFLOW AFNRJ AFQWF AFWTZ AFYQB AFZKB AGAYW AGDGC AGGBP AGMZJ AGQMX AGWZB AGYKE AHAVH AHBYD AHKAY AHSBF AHYZX AIAKS AIIXL AILAN AIMYW AITGF AJBLW AJDOV AJRNO AJZVZ AKLTO AKQUC ALFXC ALMA_UNASSIGNED_HOLDINGS AMKLP AMTXH AMXSW AMYLF AMYQR ANMIH AUKKA AXYYD AYJHY BGNMA CSCUP DNIVK DPUIP EBLON EBS EIOEI EJD ESBYG FERAY FIGPU FINBP FNLPD FRRFC FSGXE FYJPI GGCAI GGRSB GJIRD GQ6 GQ8 HF~ HMJXF HQYDN HRMNR HZ~ I0C IKXTQ IWAJR IXD IZIGR J-C JBSCW JCJTX JZLTJ KOV LLZTM M4Y NPVJJ NQJWS NU0 O9- O93 O9J PT4 RLLFE RSV S27 SCM SHX SISQX SNE SNPRN SNX SOHCF SOJ SPISZ SRMVM SSLCW STPWE T13 TSG U2A UG4 UOJIU UTJUX UZXMN VC2 VFIZW W48 Z85 ZMTXR ~A9 AACDK AAJBT AASML AAYXX ABAKF ACAOD ACDTI ACZOJ AEFQL AEMSY AFBBN AGQEE AGRTI AIGIU CITATION ROL SJYHP
ID	FETCH-LOGICAL-c249t-b0f28ed51c8f8de8a9b9fc51544e0a3671e1dea195e021032dca9dfe962d7c083
IEDL.DBID	AEJHL
ISSN	2191-1630
IngestDate	Mon Nov 04 10:22:18 EST 2024 Thu Sep 12 16:37:08 EDT 2024 Sat Dec 16 12:08:32 EST 2023
IsPeerReviewed	false
IsScholarly	true
Issue	3
Keywords	Electroporation Transformation Competent cells Bacterial growth phase Electric field strength
Language	English
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c249t-b0f28ed51c8f8de8a9b9fc51544e0a3671e1dea195e021032dca9dfe962d7c083
ORCID	0000-0002-2437-8468
PQID	2700353390
PQPubID	2043959
PageCount	5
ParticipantIDs	proquest_journals_2700353390 crossref_primary_10_1007_s12668_022_00982_2 springer_journals_10_1007_s12668_022_00982_2
PublicationCentury	2000
PublicationDate	2022-09-01
PublicationDateYYYYMMDD	2022-09-01
PublicationDate_xml	– month: 09 year: 2022 text: 2022-09-01 day: 01
PublicationDecade	2020
PublicationPlace	New York
PublicationPlace_xml	– name: New York
PublicationTitle	BioNanoScience
PublicationTitleAbbrev	BioNanoSci
PublicationYear	2022
Publisher	Springer US Springer Nature B.V
Publisher_xml	– name: Springer US – name: Springer Nature B.V
References	Zhou, S. Y., Hu, Y. J., Meng, F. C., Qu, S. Y., Wang, R., Andersen. R. J., Liao, Z. H., & Chen, M. (2018). Bacillamidins A-G from a marine-derived Bacillus pumilus. Marine drugs, 16, 9. https://doi.org/10.3390/md16090326 BlokeschMNatural competence for transformationCurrent biology: CB20162623325510.1016/j.cub.2016.11.023 Masood, S., Zhao, X. Q., & Shen, R. F. (2019). Bacillus pumilus increases boron uptake and inhibits rapeseed growth under boron supply irrespective of phosphorus fertilization. AoB Plants, 11, 4. https://doi.org/10.1093/aobpla/plz036 Black, H. F., Mastromatteo, S., Sinha, S., Ehrlich, R. L., Nislow, C., Mell, J. C. & Redfield, R. J. (2020). A competence-regulated toxin-antitoxin system in Haemophilus influenza. PLoS One, 15, 1. https://doi.org/10.1371/journal.pone.0217255 BoniferKSWenXHasimSPhillipsEKDunlapRNGannERDeBruynJMReynoldsTBBacillus pumilus B12 degrades polylactic acid and degradation is affected by changing nutrient conditionsFrontiers in microbiology201910254810.3389/fmicb.2019.02548 Huang Y., Lezyk M., Herbst F. A., Busk P. K., & Lange L. (2020). Novel keratinolytic enzymes, discovered from a talented and efficient bacterial keratin degrader. Scientific reports, 10, 1. https://doi.org/10.1038/s41598-020-66792-2 MitrofanovaOMardanovaAEvtugynVBogomolnayaLSharipovaMEffects of Bacillus serine proteases on the bacterial biofilmsBioMed research international201710.1155/2017/8525912 OpriessnigTKaruppannanAKBecklerDAit-AliTCubas-AtienzarAHalburPGBacillus pumilus probiotic feed supplementation mitigates Lawsonia intracellularis shedding and lesionsVeterinary research20195018510.1186/s13567-019-0696-1 Toimentseva, A. A., & Altenbuchner, D. (2018). Obtaining Bacillus pumilus deletion mutants for proteinase genes based on CRISPR-CAS9 technology. International research journal, 11(77), 136–142. Russian. ShaoHCaoQZhaoHTanXFengHConstruction of novel shuttle expression vectors for gene expression in Bacillus subtilis and Bacillus pumilusThe Journal of general and applied microbiology.201561412413110.2323/jgam.61.124 PerryDKuramitsuHKGenetic transformation of Streptococcus mutansInfection and immunity1981321295129710.1128/iai.32.3.1295-1297.1981 YangMMZhangWWBaiXTLiHXCenPLElectroporation is a feasible method to introduce circularized or linearized DNA into B. subtilis chromosomeMolecular biology reports2010372207221310.1007/s11033-009-9704-2 Gu, X., Sun, J., Cui, Y., Wang, X., & Sang, Y. (2019). Biological degradation of aflatoxin M1 by Bacillus pumilus E-1–1–1. Microbiology Open. 8, 3. https://doi.org/10.1002/mbo3.663 LeeNKKimWSPaikHDBacillus strains as human probiotics: Characterization, safety, microbiome, and probiotic carrierFood science and biotechnology20192851297130510.1007/s10068-019-00691-9 Zhao, H.Y., & Feng, H. (2018). Engineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution. BioMed Central Biotechnology, 18, 34. https://doi.org/10.1186/s12896-018-0451-0 VojcicLDespotovicDMartinezRMaurerK-HSchwanebergUAn efficient transformation method for Bacillus subtilis DB104Applied microbiology and biotechnology.201294248749310.1007/s00253-012-3987-2 Garcia-RamonDCBerryCTseCFernández-FernándezAOsunaAVílchezSThe parasporal crystals of Bacillus pumilus strain 15.1: A potential virulence factor?Microbial biotechnology201811230231610.1111/1751-7915.12771 Baweja, M., Tiwari, R., Singh, P. K., Nain, L. & Shukla, P. (2016). An alkaline protease from Bacillus pumilus MP 27: Functional analysis of its binding model toward its applications as detergent additive. Frontiers in microbiology, 7, 1195. https://doi.org/10.3389/fmicb.2016.01195 HaynesJABritzMLThe effect of growth conditions of Corynebacterium glutamicum on the transformation frequency obtained by electroporationJournal of general microbiology199013625526310.1099/00221287-136-2-255 McintyreDAHarlanderSKGenetic transformation of intact Lactococcus lactis subsp. lactis by high-voltage electroporationApplied and environmental microbiology19895560461010.1128/aem.55.3.604-610.1989 RahmerRMorabbi HeraviKAltenbuchnerJConstruction of a super-competent Bacillus subtilis 168 using the PmtlA-comKS inducible cassetteFrontiers in microbiology.20156143110.3389/fmicb.2015.01431 KoivVAdambergKAdambergSSumeriIKasvandikSKisandVMaivaliUTensonTMicrobiome of root vegetables-A source of gluten-degrading bacteriaApplied microbiology and biotechnology2020104208871888510.1007/s00253-020-10852-0 AuneTEVAachmannFLMethodologies to increase the transformation efficiencies and the range of bacteria that can be transformedApplied microbiology and biotechnology2010851301131310.1007/s00253-009-2349-1 SirajuddinSASundramSEvaluation of two transformation protocols and screening of positive plasmid introduction into Bacillus cereus EB2, a gram-positive bacterium using qualitative analysesBrazilian journal of microbiology202051391992910.1007/s42770-020-00241-0 ZhangNWangLWeiYEffects of Bacillus amyloliquefaciens and Bacillus pumilus on rumen and intestine morphology and microbiota in weanling Jintang black goatAnimals (Basel)2020109160410.3390/ani10091604 DowerWJMillerJFRagsdaleCWHigh efficiency transformation of E. coli by high voltage electroporationNucleic Acids Research.198816136127614510.1093/nar/16.13.6127 ShenXChenYLiuTHuXGuZDevelopment of a high-efficient transformation system of Bacillus pumilus strain DX01 to facilitate gene isolation via gfp-tagged insertional mutagenesis and visualize bacterial colonization of rice rootsFolia microbiologica.20135840941710.1007/s12223-013-0223-0 Gonzales, M. F., Brooks, T., Pukatzki, S. U. & Provenzano, D. (2013). Rapid protocol for preparation of electrocompetent Escherichia coli and Vibrio cholerae. Journal of visualized experiments: JoVE. 80, 50684. https://doi.org/10.3791/50684 Zelenikhin, P. V., Ead, Mohamed I. S., Nadyrova, A. I., Sirotkina, A. A., Ulyanova, V. V., Mironova, N. L., Mitkevich, V. A., Makarov, A. A., Zenkova, M. A., Ilinskaya, O. N. (2020). Bacillus pumilus ribonuclease inhibits migration of human duodenum adenocarcinoma HuTu 80 cells. Molekuliarnaia biologiia (Mosk), 54(1), pp.146–152. Russian. https://doi.org/10.31857/S0026898420010176 KS Bonifer (982_CR14) 2019; 10 WJ Dower (982_CR20) 1988; 16 DA Mcintyre (982_CR27) 1989; 55 982_CR19 O Mitrofanova (982_CR5) 2017 982_CR23 D Perry (982_CR25) 1981; 32 V Koiv (982_CR4) 2020; 104 TEV Aune (982_CR28) 2010; 85 JA Haynes (982_CR26) 1990; 136 L Vojcic (982_CR18) 2012; 94 N Zhang (982_CR8) 2020; 10 X Shen (982_CR22) 2013; 58 982_CR7 DC Garcia-Ramon (982_CR11) 2018; 11 982_CR3 982_CR2 982_CR1 SA Sirajuddin (982_CR16) 2020; 51 NK Lee (982_CR6) 2019; 28 982_CR12 982_CR13 982_CR15 R Rahmer (982_CR17) 2015; 6 982_CR10 MM Yang (982_CR29) 2010; 37 T Opriessnig (982_CR9) 2019; 50 H Shao (982_CR21) 2015; 61 M Blokesch (982_CR24) 2016; 26
References_xml	– ident: 982_CR15 – volume: 6 start-page: 1431 year: 2015 ident: 982_CR17 publication-title: Frontiers in microbiology. doi: 10.3389/fmicb.2015.01431 contributor: fullname: R Rahmer – ident: 982_CR3 doi: 10.1038/s41598-020-66792-2 – volume: 11 start-page: 302 issue: 2 year: 2018 ident: 982_CR11 publication-title: Microbial biotechnology doi: 10.1111/1751-7915.12771 contributor: fullname: DC Garcia-Ramon – volume: 136 start-page: 255 year: 1990 ident: 982_CR26 publication-title: Journal of general microbiology doi: 10.1099/00221287-136-2-255 contributor: fullname: JA Haynes – volume: 61 start-page: 124 issue: 4 year: 2015 ident: 982_CR21 publication-title: The Journal of general and applied microbiology. doi: 10.2323/jgam.61.124 contributor: fullname: H Shao – ident: 982_CR1 doi: 10.1186/s12896-018-0451-0 – volume: 94 start-page: 487 issue: 2 year: 2012 ident: 982_CR18 publication-title: Applied microbiology and biotechnology. doi: 10.1007/s00253-012-3987-2 contributor: fullname: L Vojcic – volume: 50 start-page: 85 issue: 1 year: 2019 ident: 982_CR9 publication-title: Veterinary research doi: 10.1186/s13567-019-0696-1 contributor: fullname: T Opriessnig – volume: 58 start-page: 409 year: 2013 ident: 982_CR22 publication-title: Folia microbiologica. doi: 10.1007/s12223-013-0223-0 contributor: fullname: X Shen – ident: 982_CR13 doi: 10.31857/S0026898420010176 – year: 2017 ident: 982_CR5 publication-title: BioMed research international doi: 10.1155/2017/8525912 contributor: fullname: O Mitrofanova – volume: 51 start-page: 919 issue: 3 year: 2020 ident: 982_CR16 publication-title: Brazilian journal of microbiology doi: 10.1007/s42770-020-00241-0 contributor: fullname: SA Sirajuddin – volume: 104 start-page: 8871 issue: 20 year: 2020 ident: 982_CR4 publication-title: Applied microbiology and biotechnology doi: 10.1007/s00253-020-10852-0 contributor: fullname: V Koiv – ident: 982_CR12 doi: 10.1002/mbo3.663 – volume: 26 start-page: 3255 issue: 23 year: 2016 ident: 982_CR24 publication-title: Current biology: CB doi: 10.1016/j.cub.2016.11.023 contributor: fullname: M Blokesch – volume: 10 start-page: 1604 issue: 9 year: 2020 ident: 982_CR8 publication-title: Animals (Basel) doi: 10.3390/ani10091604 contributor: fullname: N Zhang – ident: 982_CR19 doi: 10.3791/50684 – volume: 85 start-page: 1301 year: 2010 ident: 982_CR28 publication-title: Applied microbiology and biotechnology doi: 10.1007/s00253-009-2349-1 contributor: fullname: TEV Aune – ident: 982_CR2 doi: 10.3389/fmicb.2016.01195 – ident: 982_CR7 doi: 10.1093/aobpla/plz036 – ident: 982_CR10 doi: 10.3390/md16090326 – volume: 28 start-page: 1297 issue: 5 year: 2019 ident: 982_CR6 publication-title: Food science and biotechnology doi: 10.1007/s10068-019-00691-9 contributor: fullname: NK Lee – volume: 10 start-page: 2548 year: 2019 ident: 982_CR14 publication-title: Frontiers in microbiology doi: 10.3389/fmicb.2019.02548 contributor: fullname: KS Bonifer – volume: 32 start-page: 1295 year: 1981 ident: 982_CR25 publication-title: Infection and immunity doi: 10.1128/iai.32.3.1295-1297.1981 contributor: fullname: D Perry – volume: 37 start-page: 2207 year: 2010 ident: 982_CR29 publication-title: Molecular biology reports doi: 10.1007/s11033-009-9704-2 contributor: fullname: MM Yang – ident: 982_CR23 doi: 10.1371/journal.pone.0217255 – volume: 55 start-page: 604 year: 1989 ident: 982_CR27 publication-title: Applied and environmental microbiology doi: 10.1128/aem.55.3.604-610.1989 contributor: fullname: DA Mcintyre – volume: 16 start-page: 6127 issue: 13 year: 1988 ident: 982_CR20 publication-title: Nucleic Acids Research. doi: 10.1093/nar/16.13.6127 contributor: fullname: WJ Dower
SSID	ssj0000706383
Score	2.2821963
Snippet	Electroporation is the process of using electrical impulses to create temporary pores in the plasma membrane which, in turn, enables the penetration of nucleic...
SourceID	proquest crossref springer
SourceType	Aggregation Database Publisher
StartPage	752
SubjectTerms	Bacillus pumilus Biological and Medical Physics Biomaterials Biophysics Buffers Cell culture Cell viability Circuits and Systems Cytoplasm Deoxyribonucleic acid DNA Efficiency Electric field strength Electric pulses Electroporation Engineering Nanotechnology Nucleic acids Optimization
Title	Optimization of Electroporation Conditions for Bacillus pumilus 3–19 Strain
URI	https://link.springer.com/article/10.1007/s12668-022-00982-2 https://www.proquest.com/docview/2700353390
Volume	12
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
link	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV27TsMwFLWgXWDgjSgU5IENghK7TuyxlFQV4jEUJLbIT6miTSva7PwDf8iXYOfRAIIBpgyJLOvk-t5zfe1zATjFChmCNbPMjRKvw22eIlAn8kxIjNQKcZY3gxkMo7snehU7mRy83LpIny-qimTuqOu7bjaUUM8dPncamMizfrdpYw8hDdDsxteDm-XWirXisBDgtMvRtUHGfnld5ueBvoakmmd-K43mEae_-a-5boGNkmDCbmER22BFpztg_ZPs4C64vbd-YlJewIRTA-OiF86sNAfYm7pCtjNIaDktvORyNB5nczjLJiP3xO-vbwGDw7y_xB547McPvYFXtlXwpM21Fp7wDaJakUBSQ5WmnAlmJHGyPNrnOIwCHSjNA0a0SwgxUpIzZTQLkYqkpWz7oJFOU30AIAkj4QulqBIu9-owY5jkLgAKIgKmWuCsQjaZFeoZSa2T7EBKLEhJDlKCWqBdgZ-UK2meuMI4tpyU-S1wXqFdv_59tMO_fX4E1lD-w9zxsTZoLF4yfQxW5yo7Ke3rAy8ayXs
link.rule.ids	315,782,786,27935,27936,41075,42144,48346,48349,49651,49654,52155
linkProvider	Springer Nature
linkToHtml	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV07T8MwED5BOwADb0ShgAc2iJQ4cWKPpQ8V0ZahRWqnyIltqVJfos3Of-Af8kuw86CAYIApQyIr-nL2fZe7-w7g2hVYEVcyzdwosTyu45QIe4GlfKJiKTBn6TCYdj_oDWmjaWRyvKIXJq12L1KS6Um9bnbTvoRapvrciGBiSx-8ZaN27pWgXBuORo2PfyvajP1MgVPvRzMH2bXzfpmfF_rqk9ZE81tuNHU5rb3_vew-7OYUE9UymziADTk7hJ1PwoNH0H3UJ8U0b8FEc4Wa2TScRW4QqD43qWxjkkizWnTH4_FkkizRIpmOzdV9e3l1GOqnEyaO4anVHNTbVj5YwYp1tLWyIlthKgVxYqqokJSziKmYGGEeaXPXDxzpCMkdRqQJCV0sYs6EkszHIog1aTuB0mw-k6eAiB9EdiQEFZGJvjymFIu5cYERiRwmKnBTQBsuMv2McK2UbEAKNUhhClKIK1At0A_zvbQMTWrc1ayU2RW4LdBe3_59tbO_PX4FW-1BtxN27nsP57CN049nismqUFo9J_ICNpciucyN7R2l9s2t
linkToPdf	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3JTsMwELWglRAc2BGFAj5wg4jEqZP4WLrQCihILRK3KPEiVWrTiDZ3_oE_5EvwZGkAwQFxyiGRFY2fPW88njcInduCKGpLppmbR41GoOOUkDRcQzlUcSlIwNJmML2hO3j22h2QyVlW8ae33YuUZFbTACpN0eIqFuqqLHzTfsUz4CY6CGISQ2_CVTgW0xivNvujm-7ynEVD2snUOPXahJ7ItpnXzvw80Ff_VJLOb3nS1P10t_7_49toM6eeuJlhZQetyGgXbXwSJNxD9w96B5nmpZl4pnAn65IT50DBrRmkuAGqWLNdfB3w8WSSzHGcTMfwtN9f3yyGh2nniX301O2MWj0jb7hgcB2FLYzQVMSTglrcU56QXsBCpjgFwR5pBrbjWtISMrAYlRAq2kTwgAklmUOEyzWZO0CVaBbJQ4Sp44ZmKIQnQojKGkwpxgNwjSENLSZq6KIwsx9nuhp-qaAMRvK1kfzUSD6poXoxE36-xuY-pMxtzVaZWUOXheXL17-PdvS3z8_Q2mO769_1B7fHaJ2kcwd3zOqosnhJ5AlanYvkNMfdB6YA1kQ
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Optimization+of+Electroporation+Conditions+for+Bacillus+pumilus+3%E2%80%9319+Strain&rft.jtitle=BioNanoScience&rft.au=Danilova%2C+I.+V.&rft.au=Rudakova%2C+N.+L.&rft.au=Vasilyeva%2C+Y.+A.&rft.au=Gilmutdinova%2C+A.+I.&rft.date=2022-09-01&rft.pub=Springer+US&rft.issn=2191-1630&rft.eissn=2191-1649&rft.volume=12&rft.issue=3&rft.spage=752&rft.epage=756&rft_id=info:doi/10.1007%2Fs12668-022-00982-2&rft.externalDocID=10_1007_s12668_022_00982_2
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2191-1630&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2191-1630&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2191-1630&client=summon