Acarbose Mitigates Age-Dependent Alterations in Erythrocyte Membrane Transporters During Aging in Rats

• Published in: Rejuvenation research Vol. 26; no. 4; p. 139
• Main Authors: Arya, Jitendra Kumar, Kumar, Raushan, Singh, Akanksha, Srivastava, Parisha, Yadawa, Arun Kumar, Rizvi, Syed Ibrahim
• Format: Journal Article
• Language: English
• Published: United States 01-08-2023
• Subjects: Acarbose - pharmacology; Aging - drug effects; Aging - metabolism; Animals; Biomarkers - analysis; Biomarkers - metabolism; Erythrocyte Membrane - chemistry; Erythrocyte Membrane - drug effects; Erythrocyte Membrane - enzymology; Glycoside Hydrolase Inhibitors - pharmacology; Lipid Peroxides - analysis; Male; Osmotic Fragility - drug effects; Oxidation-Reduction - drug effects; Plasma Membrane Calcium-Transporting ATPases - analysis; Plasma Membrane Calcium-Transporting ATPases - metabolism; Protein Carbonylation - drug effects; Rats; Rats, Wistar; Sialic Acids - analysis; Sodium-Potassium-Exchanging ATPase - analysis; Sodium-Potassium-Exchanging ATPase - metabolism; Sulfhydryl Compounds - analysis; aging; membrane-bound transporters; oxidative stress; acarbose
• ISSN: 1557-8577
• DOI: 10.1089/rej.2023.0010

Acarbose (ACA), a well-studied and effective inhibitor of α-amylase and α-glucosidase, is a postprandial-acting antidiabetic medicine. The membrane of the erythrocyte is an excellent tool for analyzing different physiological and biochemical activities since it experiences a range of metabolic alterations throughout aging. It is uncertain if ACA modulates erythrocyte membrane activities in an age-dependent manner. As a result, the current study was conducted to explore the influence of ACA on age-dependent deteriorated functions of transporters/exchangers, disrupted levels of various biomarkers such as lipid hydroperoxides (LHs), protein carbonyl (PCO), sialic acid (SA), total thiol (-SH), and erythrocyte membrane osmotic fragility. In addition to a concurrent increase in Na
/H
exchanger activity and concentration of LH, PCO, and osmotic fragility, we also detected a considerable decrease in membrane-linked activities of Ca
-ATPase (PMCA) and Na
/K
-ATPase (NKA), as well as concentrations of SA and -SH in old-aged rats. The aging-induced impairment of the activities of membrane-bound ATPases and the changed levels of redox biomarkers were shown to be effectively restored by ACA treatment.