Al(OH) 3-adjuvanted vaccine-induced macrophagic myofasciitis in rats is influenced by the genetic background

• Published in: Neuromuscular disorders : NMD Vol. 16; no. 5; pp. 347 - 352
• Main Authors: Authier, François-Jérôme, Sauvat, Stéphane, Christov, Christo, Chariot, Patrick, Raisbeck, Grant, Poron, Marie-Françoise, Yiou, Françoise, Gherardi, Romain
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 2006; Elsevier
• Subjects: Adverse effects; Aluminium hydroxide; Biological Physics; Experimental model; Histopathology; Inflammatory myopathies; Lewis rat; Macrophagic myofasciitis; Morphometry; Physics; Radioisotopic study; Sprague–Dawley rat; Th1; Th2; Vaccine; Aluminium hydroxide; Inflammatory myopathies; Vaccine; Adverse effects; Macrophagic myofasciitis; Histopathology; Lewis rat; Radioisotopic study; Experimental model; Sprague–Dawley rat; Morphometry; Th1; Th2
• ISSN: 0960-8966; 1873-2364
• DOI: 10.1016/j.nmd.2006.02.004

Macrophagic myofasciitis (MMF) is a specific histopathologic lesion involved in the persistence for years of aluminum hydroxide [Al(OH)
3] at the site of previous intramuscular (i.m.) injection. In order to study mechanisms involved persistence of MMF lesions, we set up an experimental model of MMF-lesion in Sprague–Dawley and Lewis rat, by i.m. injections of 10
μL of an Al(OH)
3-adjuvanted vaccine. An evaluation carried out over a 12-month period disclosed significant shrinkage of MMF lesions with time. A radioisotopic study did not show significant aluminium uptake by Al(OH)
3-loaded macrophages. A morphometric approach showed that Lewis rats with Th1-biased immunity had significantly smaller lesions than Sprague–Dawley rats with balanced Th1/Th2 immunity. Concluding, our results indicate that genetic determinatives of cytotoxic T-cell responses could interfere with the clearance process and condition the persistence of vaccine-induced MMF-lesions.