Influence of Growth Conditions on Plasmid DNA Production

The obtention of high yields of purified plasmid DNA is viewed as an essential issue to be considered towards efficient production of DNA vaccines and therapeutic plasmids. In this work, Escherichia coli DH5α bearing the pVAX1-LacZ plasmid was grown in a developed semi-defined medium at different te...

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Bibliographic Details
Published in:	Journal of microbiology and biotechnology Vol. 19; no. 11; pp. 1408 - 1414
Main Authors:	Silva, Filomena, Universidade da Beira Interior, Avenida Infante D. Henrique, Covilha, Portugal, Passarinha, Luis, Universidade da Beira Interior, Avenida Infante D. Henrique, Covilha, Portugal, Sousa, Fani, Universidade da Beira Interior, Avenida Infante D. Henrique, Covilha, Portugal, Queiroz, Joao A., Universidade da Beira Interior, Avenida Infante D. Henrique, Covilha, Portugal, Domingues, Fernanda C., Universidade da Beira Interior, Avenida Infante D. Henrique, Covilha, Portugal
Format:	Journal Article
Language:	English
Published:	Seoul Korean Society for Applied Microbiology 01-11-2009 한국미생물·생명공학회
Subjects:	Biological and medical sciences Biotechnology Cell Culture Techniques - methods Culture Media - chemistry DNA Replication DNA, Bacterial - biosynthesis DNA, Bacterial - genetics Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - metabolism FERMENTACION FERMENTATION filamentous bacteria Fundamental and applied biological sciences. Psychology Industrial Microbiology - methods Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology microbial growth Nucleic Acid Amplification Techniques - methods Peptones - chemistry Plasmid DNA Plasmids - biosynthesis Plasmids - genetics TEMPERATURA TEMPERATURE tryptone Vaccines, DNA - biosynthesis 생물학 Plasmid Microorganism growth Temperature Growth DNA Production Bacteria filamentous bacteria Fermentation Plasmid DNA microbial growth tryptone
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