Cloning of cDNA of major antigen of foot and mouth disease virus and expression in E. coli

Double-stranded DNA copies of the single-stranded genomic RNA of foot and mouth disease virus have been cloned into the Escherichia coli plasmid pBR322. A restriction map of the viral genome was established and aligned with the biochemical map of foot and mouth disease virus. The coding sequence for...

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Bibliographic Details
Published in:	Nature (London) Vol. 289; no. 5798; pp. 555 - 559
Main Authors:	Küpper, Hans, Keller, Walter, Kurz, Christina, Forss, Sonja, Schaller, Heinz, Franze, Reinhard, Strohmaier, Karl, Marquardt, Otfried, Zaslavsky, Vladimir G, Hofschneider, Peter Hans
Format:	Journal Article
Language:	English
Published:	England 12-02-1981
Subjects:	Antigens, Viral - genetics Aphthovirus - immunology Capsid - genetics Capsid - immunology Cloning, Molecular Escherichia coli Escherichia coli - genetics Gene Expression Regulation Genes, Viral Nucleic Acid Conformation Plasmids RNA, Messenger - genetics Viral Proteins - genetics
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Summary:	Double-stranded DNA copies of the single-stranded genomic RNA of foot and mouth disease virus have been cloned into the Escherichia coli plasmid pBR322. A restriction map of the viral genome was established and aligned with the biochemical map of foot and mouth disease virus. The coding sequence for structural protein VP1, the major antigen of the virus, was identified and inserted into a plasmid vector where the expression of this sequence is under control of the phage lambda PL promoter. In an appropriate host the synthesis of antigenic polypeptide can be demonstrated by radioimmunoassay.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0028-0836 1476-4687
DOI:	10.1038/289555a0