Effects of Insulin-Like Growth Factor 1 on the In Vitro Development of Mouse Embryos after Cryopreservation

• Published in: Russian journal of developmental biology Vol. 52; no. 2; pp. 120 - 124
• Main Authors: Brusentsev, E. Yu, Kizilova, E. A., Igonina, T. N., Ranneva, S. V., Amstislavsky, S. Ya
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-03-2021; Springer Nature B.V
• Subjects: Animal Anatomy; Biomedical and Life Sciences; Blastocysts; Cell culture; Cryopreservation; Cryoprotectors; Developmental Biology; Developmental stages; DNA fragmentation; Embryos; Gametes; Genomes; Histology; Insulin; Insulin-like growth factor I; Insulin-like growth factors; Life Sciences; Morphology; Propylene glycol; Short Communications; IGF-1; preimplantation embryos; mice; cryopreservation; in vitro culture
• ISSN: 1062-3604; 1608-3326
• DOI: 10.1134/S1062360421020028

Cryopreservation of gametes and embryos is widely used for mammalian genome resource banking. Effects of insulin-like growth factor-1 (IGF-1) on the in vitro development of cleavage stage CD1 mouse embryos after their cryopreservation were studied. The mouse embryos developed in vivo up to the 4–6-cell stage were frozen in a programmable freezer according to standard protocol with 10% propylene glycol as a cryoprotectant and thereafter were thawed and cultured in vitro in KSOM for 48 h. The percentage of embryos developed up to blastocyst stage in culture medium supplemented with IGF-1 (40 ng/mL) was higher as compared to controls (78.9 and 47.4%, respectively,
p
< 0.01). Moreover, blastocysts developed in the presence of IGF-1 contained more cells as compared to controls (79.8 ± 3.9 and 58.6 ± 3.6 cells, respectively,
p
< 0.001). However, the index of nuclear fragmentation did not differ between these groups. Experimental results presented here revealed the stimulating effects of IGF-1 on the mouse preimplantation embryos after their cryopreservation.