Revealing tick diversity: Chemical profiling and dynamics in scanning microscopy and molecular phylogenetics

• Published in: Microscopy research and technique Vol. 87; no. 11; pp. 2590 - 2602
• Main Authors: Malik, Ayesha, Afshan, Kiran, Okla, Mohammad K., Saleh, Ibrahim A., Razzaq, Abdul, Hussain, Munib, Firasat, Sabika, Lika, Erinda, Fuentes, Màrius Vicent
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01-11-2024; Wiley Subscription Services, Inc
• Subjects: Animals; Chemical composition; chromatogram; Chromatography; Disease control; Ecological adaptation; Ecological studies; Endemic species; Exoskeleton; Exoskeletons; Female; Goats; High performance liquid chromatography; Hydrocarbons; Ixodidae; Liquid chromatography; microscopy; Microscopy, Electron, Scanning; Phylogenetics; Phylogeny; Rhipicephalus; Rhipicephalus - classification; Rhipicephalus - genetics; RNA, Ribosomal, 16S - genetics; rRNA 16S; Scanning; Scanning microscopy; Sheep; Sheep Diseases - parasitology; Tick Infestations - veterinary; Ticks; microscopy; hydrocarbons; Ixodidae; chromatography; chromatogram
• ISSN: 1059-910X; 1097-0029; 1097-0029
• DOI: 10.1002/jemt.24620

This study presents a comprehensive investigation into the evolutionary trajectories of Rhipicephalus ticks (Ixodidae) through the interpretation of molecular phylogenetics, elucidating their chromatographic spectrum. The use of advanced chromatographic tools in this study explored the dynamics chemical profiling, providing valuable insights into the evolutionary history and ecological adaptations. Prevalence of Rhipicephalus ticks was 4.5% in sheep and 3.9% in goats. The ITS2 sequence of the Rhipicephalus sanguineus (OK642408) and Rhipicephalus microplus (OK642409) form a distinct clade with sequences from other countries. The 16S rRNA sequences of R. sanguineus (OK560870) clustered with sequences form three lineages, tropical, temperate, and south‐eastern. The Cox I gene‐identified Rhipicephalus turanicus (OK623472) and R. microplus (OK623463) form separate clades with sequences. The HPLC chromatogram of tick samples reveals a diverse array of identified hydrocarbons, explained the complex chemical composition of their exoskeletons. This analytical approach provides valuable insights into the specific hydrocarbon profiles, allowing for potential applications in species differentiation, ecological studies, and a deeper understanding of the functional roles played by hydrocarbon compounds in tick physiology. The findings revealed the potential of applying molecular phylogenetics tools with chromatography not only to enhance our understanding of tick evolution but also to inform strategies for disease control and management in regions where Rhipicephalus ticks (Ixodidae) are endemic.
Research Highlights
Chemical mapping utilizing advanced chromatographic techniques.
Scanning microscopic insights high‐resolution scanning tool to observe structural and morphological features of ticks at a molecular level.
Molecular phylogeny data elucidate the evolutionary relationships among tick species.
Investigating HPLC chromatograms for morpho‐scanning electron microscope authentication of Rhipicephalus ticks.