Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs

Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs

Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH4NO3, MnSO4⋅7H2O, Soya bean cake, and K2HPO4 were found to be most si...

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Bibliographic Details
Published in:Biotechnology research international Vol. 2011; no. 2011; pp. 1 - 7
Main Authors: Dilipkumar, M., Rajasimman, M., Rajamohan, N.
Format: Journal Article
Language:English
Published: Cairo, Egypt Hindawi Puplishing Corporation 30-03-2011
SAGE-Hindawi Access to Research
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Summary:Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH4NO3, MnSO4⋅7H2O, Soya bean cake, and K2HPO4 were found to be most significant nutrient components. Hence, these 4 components are selected. The selected components were optimized using response surface methodology (RSM). The optimum conditions are NH4NO3—6.63 mg/gds, MnSO4⋅7H2O—26.16 mg/gds, Soya bean cake—60.6 mg/gds, and K2HPO4—5.24 mg/gds. Under these conditions, the production of inulinase was found to be 76 U/gds.
Bibliography:ObjectType-Article-1
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Academic Editor: Triantafyllos Roukas
ISSN:2090-3138
2090-3146
DOI:10.4061/2011/708043