Use of random amplified polymorphic DNA (RAPD) to characterize race 2 of Fusarium oxysporum f. sp. pisi

• Published in: Phytopathology Vol. 83; no. 6; pp. 612 - 614
• Main Authors: Grajal-Martin, M.J, Simon, C.J, Muehlbauer, F.J
• Format: Journal Article
• Language: English
• Published: St. Paul, MN American Phytopathological Society 01-06-1993
• Subjects: ADN; Agronomy. Soil science and plant productions; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; FUSARIUM OXYSPORUM; GENETICA; GENETIQUE; PATHOTYPE; PATOTIPOS; Phytopathology. Animal pests. Plant and forest protection; PISUM SATIVUM; POLIMORFISMO GENETICO; POLYMORPHISME GENETIQUE; VARIACION GENETICA; VARIATION GENETIQUE; Variation, races, biotypes, parasitic specialization, genetics; WASHINGTON; Fusarium oxysporum; Genetic variability; Plant pathogen; Diversity; Physiologic race; Fungi; Characterization; DNA; Fingerprint method; RAPD markers; Isolate; Fungi Imperfecti; Technique; Molecular biology; Thallophyta
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/Phyto-83-612

Genetic variability within four races of Fusarium oxysporum f. sp. pisi was assessed by random amplified polymorphic DNA (RAPD). Banding patterns generated from isolates of race 2 by 14 selected primers were uniform relative to patterns generated from races 1, 5, and 6. Isolates of races 1, 5, and 6 showed much greater variability within each race than did isolates of race 2. The high level of genetic variability among and within races of F. o. pisi allowed for fingerprinting of most of the isolates by examining patterns produced by several primers. Generalized race-specific patterns were not found for races 1, 5, and 6. The results suggested that races 1, 5, and 6 be grouped separately from race 2. Similarities and differences in banding patterns obtained by RAPD could be a useful molecular tool in evolutionary studies of the origins of different races