Local actions of angiotensin II: quantitative in vitro autoradiographic localization of angiotensin II receptor binding and angiotensin converting enzyme in target tissues

• Published in: Journal of cardiovascular pharmacology Vol. 8 Suppl 10; p. S35
• Main Authors: Chai, S Y, Allen, A M, Adam, W R, Mendelsohn, F A
• Format: Journal Article
• Language: English
• Published: United States 1986
• Subjects: Adrenal Cortex - metabolism; Angiotensin II - metabolism; Animals; Autoradiography; Brain - metabolism; Iodine Radioisotopes; Kidney - metabolism; Peptidyl-Dipeptidase A - metabolism; Rats; Rats, Inbred Strains; Receptors, Angiotensin - metabolism
• ISSN: 0160-2446
• DOI: 10.1097/00005344-198600101-00008

In order to gain insight into the local actions of angiotensin II (ANG II) we have determined the distribution of a component of the effector system for the peptide, the ANG II receptor, and that of an enzyme-catalysing ANG II formation, angiotensin converting enzyme (ACE), by in vitro autoradiography in several target tissues. The superagonist ANG II analog, 125I[Sar1]ANG II, or the antagonist analog, 125I[Sar1,Ile8]ANG II, were used as specific radioligands for ANG II receptors. A derivative of the specific ACE inhibitor, lysinopril, called 125I-351A, was used to label ACE in tissues. In the adrenal, a high density of ANG II receptors occurs in the glomerulosa zone of the cortex and in the medulla. ACE is also localized in these two zones, indicating that local production of ANG II may occur close to its sites of action in the zona glomerulosa and adrenal medulla. In the kidney, a high density of ANG II receptors is associated with glomeruli in the cortex and also with vasa recta bundles in the inner stripe of the outer medulla. ACE is found in very high concentration in deep proximal convoluted tubules of the cortex, while much lower concentrations of the enzyme occur in the vascular endothelium throughout the kidney. In the central nervous system three classes of relationships between ANG II receptors and ACE are observed: In the circumventricular organs, including the subfornical organ and organum vasculosum of the lamina terminalis, a high concentration of both components occurs. Since these structures have a deficient blood-brain barrier, local conversion of circulating angiotensin I (ANG I) to ANG II may contribute to the action of ANG II at these sites.