Activity patterns of cardiomyocytes in electrospun scaffolds of poly (ε-caprolactone), collagen, and epicatechin

• Published in: Materials today communications Vol. 31; p. 103405
• Main Authors: Miranda-Buendia, E., González-Gómez, G.H., Falcón-Neri, M.A., Durán-Pastén, M.L., Jiménez-Martínez, C., Vera-Graziano, R., Ospina-Orejarena, A., Rivera-Torres, F., Prado-Villegas, G., Maciel-Cerda, A.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-06-2022
• Subjects: Activity patterns; Chick embryonic cardiomyocytes; Collagen; Electrospun scaffolds; Epicatechin; Poly (ε-caprolactone); Poly (ε-caprolactone); Electrospun scaffolds; Chick embryonic cardiomyocytes; Activity patterns; Collagen; Epicatechin
• ISSN: 2352-4928; 2352-4928
• DOI: 10.1016/j.mtcomm.2022.103405

Cardiovascular pathologies can be treated with a substrate that mimics the extracellular matrix (ECM) to regenerate infarcted tissue. We determined the activity patterns of chick embryonic cardiomyocytes in electrospun scaffolds produced with combinations of poly (ε-caprolactone) (PCL), collagen (COL), and epicatechin (EPI); namely, PCL, PCL-EPI, PCL-COL, and PCL-COL-EPI, and with the cytotoxicity assay and calcium imagine proved their effectiveness at keeping cardiomyocyte cells alive. The addition of collagen with the same electrospun parameters was found to decrease fiber diameter, Young’s modulus, and contact angle, while the chick embryonic cardiomyocytes presented a higher frequency of activity and synchronicity. In contrast, the scaffolds comprised only of PCL and PCL-EPI showed a higher Young’s modulus, a larger fiber diameter, a greater contact angle. Besides, chick cardiomyocytes did not present synchronicity and had a lower frequency. The activity patterns of chick cardiomyocytes could be modified with the addition collagen or epicatechin in the PCL scaffolds for the potential application to regenerate damaged cardiac tissue.
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•Activity of cardiomyocytes is influenced by properties of the scaffolds.•Epicatechin decreases frequency the activity pattern of chick cardiomyocytes.•Smaller Young´s module in scaffoldings allow better frequency and synchronicity.