A fluorescent NBD “turn-on” probe for the rapid and on-site analysis of fructose in food

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 320; p. 124612
• Main Authors: Chen, Yanan, Li, Yajing, Wang, Wenjing, Jiang, Long, Yin, Shaojie, Guo, Ziwei, Wu, Wenyan, Wang, Chongqing, Lu, Sheng, Wang, Fang, Chen, Xiaoqiang
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 05-11-2024
• Subjects: Electrospinning technique; Fluorescent probe; Fructose; Metabolic disease; Monitoring; Metabolic disease; Electrospinning technique; Fructose; Monitoring; Fluorescent probe
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2024.124612

[Display omitted]
•A new fluorescence turn-on probe for determining fructose is synthesized.•This fructose sensor exhibits new binding modes.•The binding of probe to fructose occurs quickly.•This strategy is successfully used for analysis of fructose in food.•NBD-PQ-B/PEO fiber film realizes on-site detection of fructose conveniently.
High fructose intake is an important cause of metabolic disease. Due to the increasing prevalence of metabolic diseases worldwide, the development of an accurate and efficient tool for monitoring fructose in food is urgently needed to control the intake of fructose. Herein, a new fluorescent probe NBD-PQ-B with 7-nitrobenz-2-oxa-1, 3-diazole (NBD) as the fluorophore, piperazine (PQ) as the bridging group and phenylboronic acid (B) as the recognition receptor, was synthesized to detect fructose. The fluorescence of NBD-PQ-B increased linearly at 550 nm at an excitation wavelength of 497 nm with increasing fructose concentration from 0.1 to 20 mM. The limit of detection (LOD) of fructose was 40 μM. The pKa values of NBD-PQ-B and its fructose complexes were 4.1 and 10.0, respectively. In addition, NBD-PQ-B bound to fructose in a few seconds. The present technique was applied to determine the fructose content in beverages, honey, and watermelon with satisfactory results. Finally, the system could not only be applied in an aqueous solution with a spectrophotometer, but also be fabricated as a NBD-PQ-B/polyvinyl oxide (PEO) film by electrospinning for on-site food analysis simply with the assistance of a smartphone.