Pediatric reference values of NT-proBNP and Galectin-3 based on a French cohort

• Published in: Clinica chimica acta Vol. 564; p. 119925
• Main Authors: Gravrand, Victor, Lefebvre, Corentin S., Hamza, Fatma, Della-Negra, Thibaud, Coyaud, Vincent, Vasseur, Axelle, Hennequin, Carole, Nivet-Antoine, Valérie, Schaffner, Damien
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-01-2025
• Subjects: Cardiac biomarkers; Galectin-3; NT-proBNP; Pediatric reference values; CRP; Gal3; NT-proBNP; Cardiac biomarkers; LLOD; WBC; Galectin-3; Pediatric reference values
• ISSN: 0009-8981; 1873-3492; 1873-3492
• DOI: 10.1016/j.cca.2024.119925

•In pediatric cardiology, some new biomarkers have assay-specific normal values in addition to age and sex.•Normal values are established on large, healthy pediatric cohorts.•Using the Abbott immunoassay system, we present pediatric age-adjusted normative values for NT-proBNP.•Using the Abbott immunoassay system, we present, for the first time, age-adjusted normative values for Galectin-3.
In pediatric cardiology, the fact that some new biomarkers have assay-specific normal values has to be considered for correct clinical decisions. The current study aimed to provide age-adjusted normative values for NT-proBNP and Galectin-3 using the Abbott immunoassay system from a prospective French pediatric cohort sera collection and to validate our data for NT-proBNP on a second retrospective cohort.
We analyzed 283 consecutive samples for NT-proBNP and 140 samples for Galectin-3 collected from apparently healthy children (0–18 years) with outpatient treatment at our institution (Hôpital Necker-Enfants malades, Paris, France) during 24 months.
For NT-proBNP and Galectin-3, we establish four age partitions, respectively two (<2 years / >2 years) and establish upper reference values and their 90 % CI for each biomarker (Galectin-3 (ng/mL): 56 [44–70] / 26 [23–29]). We evaluated the diagnostic performance of our upper reference values of NT-proBNP on a retrospective cohort (n = 428) with positive predictive value of 0.92.
Using Abbott immunoassay system, we report age-specific reference values for NT-proBNP and for the first time for Galectin-3 in a healthy French pediatric cohort. These data call for larger cohort studies to define more robustly percentiles and diagnostic performance for NT-proBNP.