Fungicide Resistance and Host Influence on Population Structure in Botrytis spp. from Specialty Crops in California

Fungicide Resistance and Host Influence on Population Structure in Botrytis spp. from Specialty Crops in California

is an important genus of plant pathogens causing pre- and postharvest disease on diverse crops worldwide. This study evaluated isolates collected from strawberry, blueberry, and table grape berries in California. Isolates were evaluated for resistance to eight different fungicides, and 60 amplicon m...

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Published in:Phytopathology Vol. 112; no. 12; pp. 2549 - 2559
Main Authors: Naegele, Rachel P, Abdelsamad, Noor, DeLong, Jeff A, Saito, Seiya, Xiao, Chang-Lin, Miles, Timothy D
Format: Journal Article
Language:English
Published: United States 01-12-2022
Subjects:
Botrytis - genetics
California
Drug Resistance, Fungal - genetics
Fragaria
Fungicides, Industrial - pharmacology
Plant Diseases
California
fungal pathogens
genetics
population biology
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Abstract is an important genus of plant pathogens causing pre- and postharvest disease on diverse crops worldwide. This study evaluated isolates collected from strawberry, blueberry, and table grape berries in California. Isolates were evaluated for resistance to eight different fungicides, and 60 amplicon markers were sequenced (neutral, species identification, and fungicide resistance associated) distributed across 15 of the 18 . chromosomes. Fungicide resistance was common among the populations, with resistance to pyraclostrobin and boscalid being most frequent. Isolates from blueberry had resistance to the least number of fungicides, whereas isolates from strawberry had resistance to the highest number. Host and fungicide resistance-specific population structure explained 12 and 7 to 26%, respectively, of the population variability observed. Fungicide resistance was the major driver for population structure, with select fungicides explaining up to 26% and multiple fungicide resistance explaining 17% of the variability observed. Shared and unique significant single-nucleotide polymorphisms (SNPs) associated with host and fungicide (fluopyram, thiabendazole, pyraclostrobin, and fenhexamid) resistance-associated population structures were identified. Although overlap between host and fungicide resistance SNPs were detected, unique SNPs suggest that both host and fungicide resistance play an important role in population structure.
AbstractList Botrytis is an important genus of plant pathogens causing pre- and postharvest disease on diverse crops worldwide. This study evaluated Botrytis isolates collected from strawberry, blueberry, and table grape berries in California. Isolates were evaluated for resistance to eight different fungicides, and 60 amplicon markers were sequenced (neutral, species identification, and fungicide resistance associated) distributed across 15 of the 18 B. cinerea chromosomes. Fungicide resistance was common among the populations, with resistance to pyraclostrobin and boscalid being most frequent. Isolates from blueberry had resistance to the least number of fungicides, whereas isolates from strawberry had resistance to the highest number. Host and fungicide resistance-specific population structure explained 12 and 7 to 26%, respectively, of the population variability observed. Fungicide resistance was the major driver for population structure, with select fungicides explaining up to 26% and multiple fungicide resistance explaining 17% of the variability observed. Shared and unique significant single-nucleotide polymorphisms (SNPs) associated with host and fungicide (fluopyram, thiabendazole, pyraclostrobin, and fenhexamid) resistance-associated population structures were identified. Although overlap between host and fungicide resistance SNPs were detected, unique SNPs suggest that both host and fungicide resistance play an important role in Botrytis population structure.
is an important genus of plant pathogens causing pre- and postharvest disease on diverse crops worldwide. This study evaluated isolates collected from strawberry, blueberry, and table grape berries in California. Isolates were evaluated for resistance to eight different fungicides, and 60 amplicon markers were sequenced (neutral, species identification, and fungicide resistance associated) distributed across 15 of the 18 . chromosomes. Fungicide resistance was common among the populations, with resistance to pyraclostrobin and boscalid being most frequent. Isolates from blueberry had resistance to the least number of fungicides, whereas isolates from strawberry had resistance to the highest number. Host and fungicide resistance-specific population structure explained 12 and 7 to 26%, respectively, of the population variability observed. Fungicide resistance was the major driver for population structure, with select fungicides explaining up to 26% and multiple fungicide resistance explaining 17% of the variability observed. Shared and unique significant single-nucleotide polymorphisms (SNPs) associated with host and fungicide (fluopyram, thiabendazole, pyraclostrobin, and fenhexamid) resistance-associated population structures were identified. Although overlap between host and fungicide resistance SNPs were detected, unique SNPs suggest that both host and fungicide resistance play an important role in population structure.
Author DeLong, Jeff A
Naegele, Rachel P
Xiao, Chang-Lin
Saito, Seiya
Abdelsamad, Noor
Miles, Timothy D
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  organization: Michigan State University, Plant Pathology, 426 Auditorium Road, East Lansing, MI 48824
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Snippet is an important genus of plant pathogens causing pre- and postharvest disease on diverse crops worldwide. This study evaluated isolates collected from...
Botrytis is an important genus of plant pathogens causing pre- and postharvest disease on diverse crops worldwide. This study evaluated Botrytis isolates...
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SubjectTerms Botrytis - genetics
California
Drug Resistance, Fungal - genetics
Fragaria
Fungicides, Industrial - pharmacology
Plant Diseases
Title Fungicide Resistance and Host Influence on Population Structure in Botrytis spp. from Specialty Crops in California
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