Hormones in Foods: Presence of Enterostatin-Like Immunoreactivities in Bovine Milk

• Published in: Nutritional neuroscience Vol. 2; no. 3; pp. 147 - 154
• Main Authors: Prasad, Chandan, Debata, Chittaranjan, McGregor, John U.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01-01-1999
• Subjects: Ala-Pro-Gly-Pro-Arg; Cow's milk; Enterostatins; Val-Pro-Asp-Pro-Arg; Val-Pro-Gly-Pro-Arg; Val-Pro-Gly-Pro-Arg; Val-Pro-Asp-Pro-Arg; Cow's milk; Enterostatins; Ala-Pro-Gly-Pro-Arg
• ISSN: 1028-415X; 1476-8305
• DOI: 10.1080/1028415X.1999.11747273

Enterostatins, pentapeptides (Val-Pro-Asp-Pro-Arg [VPDPR], Val-Pro-Gly-Pro-Arg, Ala-Pro-Gly-Pro- Arg [APGPR], and others) derived from the amino terminus of procolipase, are endogenous to a variety of tissues and body fluids including brain, gut, blood, cerebrospinal fluid, and urine. The administration of exogenous peptides has been shown to elicit a variety of biologic activities, including a decrease in dietary fat preference and pancreatic insulin secretion. Since milk is a rich source of a variety of bioactive substances, especially peptides, we investigated the presence of enterostatin-like immunoreactivity in bovine milk. We measured enterostatins-APGPR and VPDPR-in milk from a herd of 19 cows randomly selected from the Louisiana State University Department of Dairy Science Research Herd in Baton Rouge; the results of this study show a mean peptide concentration in raw milk of 33.7 ± 2.9 ng/ml for APGPR and of 104.5 ± 16.3 ng/ml for VPDPR. A further chromatographic characterization of the nature of APGPR- and VPDPR-like immunoreactivities suggested the endogenous peptides share a common epitope with APGPR or VPDPR but are not APGPR or VPDPR. Unlike APGPR or VPDPR, the endogenous peptides were heat-labile and therefore their values were much lower in pasteurized milk.