Solubilisation & purification of membrane proteins using benzylamine-modified SMA polymers

Extraction of proteins from the membrane using styrene maleic acid co-polymers (SMA), forming SMA lipid particles (SMALPs), has allowed for the first time the purification of membrane proteins with their lipid bilayer environment. To date, SMA2000 has been the most effective polymer used for this pu...

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Bibliographic Details
Published in:	Biophysical chemistry Vol. 316; p. 107343
Main Authors:	Akram, Aneel, Hadasha, Waled, Kuyler, Gestél C., Smith, Michael-Phillip, Bailey-Dallaway, Shauna, Preedy, Aiden, Browne, Caolan, Broadbent, Luke, Hill, Adam, Javaid, Tahreem, Nazar, Haroon, Samra, Nikita, Naveed, Anadil, Tregunna, Holly, Joshi, Hetal, Akhtar, Nusheen, Javed, Aneesa, Bowater, Jessica, Ravenhill, Joel, Hajdu, Patrik, Ali, Yazdan, Tailor, Yanik, Mumtaz, Sabreen, Hamza, Mohammed, Gill, Kiran, Gillett, Jemma, Patton, Faye, Arshid, Huma, Zaheer, Maria, Qureshi, Hannah, Edwards, Isabel, Patel, Shreya, Azadi, Aaminah, Pollock, Naomi, Kitchen, Philip, Klumperman, Bert, Rothnie, Alice J.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-01-2025
Subjects:	Atm1 Detergent-free Mass photometry Nanoparticle SMALP Mn Nanoparticle CD81 BPT E.coli AQP4 DLS ZipA Ð BSA DIBMA THF PDI SMAnh FTIR SMA-BA 7 kDa RAFT DMF DMPC SMALP SMA1000-BA GSSG SMA Detergent-free Sf9 SMA-BA 2 kDa Atm1 AIBN MAnh MRP4 P.pastoris SEC SDS-PAGE MEK SMA-BA 4 kDa LB Mass photometry ABC transporter Ag-GSH Ni-NTA ATR BA
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