Induction of haploid rice plants through in vitro anther culture

• Published in: Pakistan journal of biological sciences Vol. 6; no. 14; pp. 1250 - 1252
• Main Authors: Shahnewaz, S, Bari, M.A, Siddique, N.A, Khatun, N, Rahman, M.H, Haque, M.E. (Rajshahi Univ. (Bangladesh). Plant Biotechnology Lab.)
• Format: Journal Article
• Language: English
• Published: 01-07-2003
• Subjects: 2,4-D; ANA; ANTHER CULTURE; CITOQUININAS; CULTIVO DE ANTERAS; CULTURE D'ANTHÈRE; CULTURE MEDIA; CYTOKININE; CYTOKININS; HAPLOID; IN VITRO REGENERATION; MEDIO DE CULTIVO; MILIEU DE CULTURE; NAA; ORYZA SATIVA; REGENERACIÓN IN VITRO; RÉGÉNÉRATION IN VITRO; VARIEDADES; VARIETIES; VARIÉTÉ
• ISSN: 1028-8880
• DOI: 10.3923/pjbs.2003.1250.1252

Anthers of three rice cultivars having late uninucleate microspores were studied for induction of haploid rice plants. Anthers were cultured on Z2 medium supplemented with various combinations and concentrations of auxins and kinetin (cytokinins). The best callusing from cultured anthers obtained in Z2 medium containing 2,4-D 2 mg L-1, NAA 2.5 mg L-1 and kin 0.5 mg L-1. For regeneration of haploid plantlets anther derived calli were transferred to modified MS medium enriched with kin 1 mg L-1, NAA 1 mg L-1, BAP 1.0 ml L-1 and incubated at 25 plus minus 1 degree centigrade in light. BRRI Dhan-29 produced both green and albino plants while BR-3 produced green plants only. Green plantlets obtained from BRRI Dhan-29 were examined and found to be 69.2% haploid plants.