Clostridium weizhouense sp. nov., an anaerobic bacterium isolated from activated sludge of petroleum wastewater

• Published in: International journal of systematic and evolutionary microbiology Vol. 72; no. 4
• Main Authors: Li, Qing, Shen, Taiming, Dong, Yingying, Liu, Yuhui, Nong, Jieliang, Pan, Jiayuan, Fu, Bei, Xie, Qinglin
• Format: Journal Article
• Language: English
• Published: England 01-04-2022
• Subjects: Clostridium; new species; taxonomy; anaerobic bacteria
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.005330

A Gram-stain-positive, anaerobic, spore-forming, rod-shaped (0.4-0.6 µm×2.5-3.2 µm), flagellated bacterium, designated strain YB-6
, was isolated from activated sludge of an anaerobic tank at Weizhou marine oil mining wastewater treatment plant in Beihai, Guangxi, PR China. The culture conditions were 25-45 °C (optimum, 37 °C), pH 4-12 (pH 7.0) and NaCl concentration of 0-7 % w/v (0%). Strain YB-6
grew slowly in petroleum wastewater and removed 68.2 % of the total organic carbon in petroleum wastewater within 10 days. Concentrations of naphthalene, anthracene and phenanthrene at an initial concentration of 50 mg l
were reduced by 32.8, 40.4 and 14.6 %, respectively, after 7 days. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain YB-6
belongs to
cluster I and is most closely related to
CK55
(98.5 % similarity). The genome size of strain YB-6
was 3.96 Mb, and the G+C content was 26.5 mol%. The average nucleotide identity value between strain YB-6
and
CK55
was 81.9 %. The major fatty acids in strain YB-6
were C
FAME, C
FAME and summed feature 4 (unknown 14.762 and/or C
FAME). The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five unidentified aminophospholipids, one unidentified glycolipid and one unidentified aminolipid. Diaminopimelic acid was not detected in the strain YB-6
cell walls. Whole-cell sugars mainly consisted of ribose and galactose. Based on the results of phenotypic and genotypic analyses, strain YB-6
represents a novel species of the genus
, for which the name
sp. nov. is proposed. The type strain is YB-6
(=GDMCC 1.2529
=JCM 34754
).