Differential expression of calcium-dependent protein kinase genes (CDPK1–14) in Rubia cordifolia callus cultures transformed with the rolB and rolC genes

• Published in: Plant gene Vol. 21; p. 100215
• Main Authors: Shkryl, Y.N., Veremeichik, G.N., Silantieva, S.A., Bulgakov, V.P.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-03-2020
• Subjects: Agrobacterium rhizogenes; Ca2+-dependent activity; Plant cell cultures; Plast genes; Protein kinase; T-DNA integration and expression; Agrobacterium rhizogenes; Plant cell cultures; T-DNA integration and expression; Plast genes; Ca2+-dependent activity; Protein kinase
• ISSN: 2352-4073; 2352-4073
• DOI: 10.1016/j.plgene.2019.100215

Integration of Agrobacterium rhizogenes T-DNA region leads to multiple changes in the physiology and biochemistry of the transformed plant cells. We have previously shown that one of these possible effects may be related to the changes in the expression profile of calcium-dependent protein kinase (CDPK) genes. Since the key determinants of T-DNA are rol genes, in this work, we studied the individual action of the rolB and rolC genes on the expression of CDPK genes in transgenic cells of Rubia cordifolia calli. Rol genes caused a general increase in total R. cordifolia CDPK transcripts abundance. Expression of RcCDPK2, RcCDPK7, RcCDPK8, RcCDPK9 and RcCDPK11 was 1.3- to 3.5-fold higher in both rolB- and rolC-transformed cells than in non-transformed cells, whereas expression of RcCDPK10 was 1.9-fold lower. Expression of RcCDPK1, RcCDPK13 and RcCDPK14 was up-regulated by the rolB gene, and the transcriptional level of RcCDPK4 and RcCDPK5 was higher in the rolC-expressing cells. Our results indicate that complex but often divergent effects of rolB and rolC on transformed plant cells may be attributed to changes caused by these genes on particular CDPK isoforms.
•The rol genes increase the mean of total CDPK transcript abundance in transgenic cells•The rolB and rolC differently regulate CDPK genes responsive to abiotic stresses•Divergent effects of rol genes may be attributed to changes of particular CDPK isoforms•Rol genes modulate CDPK isoforms implicated in ROS production and detoxification•Thus, pRiA4-induced ROS suppression is likely attributed with rol-induced CDPK changes