Comparative investigation of different methods of storage of lactic acid bacteria

• Published in: Microbiology (New York) Vol. 69; no. 1; pp. 83 - 88
• Main Authors: Stoyanova, L. G., Arkad’eva, Z. A.
• Format: Journal Article
• Language: English
• Published: New York Springer Nature B.V 01-01-2000
• Subjects: Freeze drying; Lactic acid bacteria; Mineral oils; Organic acids; Skim milk
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1007/BF02757262

The study was undertaken to elucidate how different methods of storage (immersing in mineral oil, lyophilization, and subculturing) of lactic acid bacteria belonging to the generaLactobacillus andLactococcus affect their viability, antibiotic activity, and ability to accumulate organic acids. Storage of the lactic acid bacteriumLactococcus lactis subsp.lactis by immersion in mineral oil proved to be ineffective. Lyophilization allowed the survival of a sufficiently large number of cells, although their antibiotic activity somewhat decreased. The resuscitation of lyophilized bacteria by subculturing them in rich nutrient media, such as skim milk, led to the restoration of their physiological activity, including the effective antimicrobial spectrum