Immunohistochemical features of the bone tissue of the lower extremities in patients with type 2 diabetes mellitus

• Published in: Sakharnyĭ diabet Vol. 24; no. 5; pp. 448 - 455
• Main Authors: Tokmakova, A. Yu, Kogan, E. A., Zaitseva, E. L., Demura, S. A., Zharkov, N. V., Kalandiya, M. M., Galstyan, G. R.
• Format: Journal Article
• Language: English
• Published: Endocrinology Research Centre 04-01-2022
• Subjects: age; bone metabolism; diabetic foot; immunohistochemistry; markers; morphology; neuroosteoarthropathy; type 2 diabetes
• ISSN: 2072-0351; 2072-0378
• DOI: 10.14341/DM12812

Background
: Diabetic neuroosteoarthropathy is a serious disabling complication of diabetes mellitus, which, in the absence of timely correct treatment, can lead to high amputations of the affected limb. At present, the reasons and mechanism of the development of Charcot’s foot are not completely clear. It is extremely important to determine the pathophysiological mechanisms of DNOAP formation and to search for reliable markers-predictors of this pathology.
Aim:
To study the immunohistochemical characteristics of the bone tissue of the lower extremities in patients with diabetic neuroosteoarthropathy in comparison with patients with diabetes mellitus without this pathology.
Materials and methods
: During the foot surgery, a bone fragment of the foot was harvested for immunohistochemical study of receptor markers for PINP, PIIINP, and RAGE in the group of patients with DNOAP compared with the control group.
Results:
The study included 20 patients with type 2 diabetes mellitus and were divided into 2 groups: 10 patients with DNOAP made up group 1, 10 patients without DNOAP — group 2.
Patients in both groups were comparable in AGE, experience with type 2 diabetes, and glycemic control.
During the immunohistochemical study, a significant increase in the staining intensity of receptor markers for PINP, PIIINP, and AGE was recorded in the group of patients with DNOAP compared with the control group (p <0.05).
Conclusion:
For the first time, an immunohistochemical study of markers of bone resorption and AGE was carried out in persons with DNOAP. The results obtained indicate impaired collagen formation and, as a consequence, impaired bone formation and bone resorption in patients with DNOAP: in group 1, a statistically significant increase in the expression of PINP, PIIINP, and RAGE was revealed.