Lectin-binding analysis of the biofilm exopolymeric matrix carbohydrate composition of corrosion-aggressive bacteria

• Published in: Prikladnaja biohimija i mikrobiologija Vol. 49; no. 5; p. 450
• Main Authors: Purish, L M, Asaulenko, L G, Abdulina, D R, Voĭtchuk, S I, Iutinskaia, G A
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-09-2013
• Subjects: Bacteria - chemistry; Bacteria - metabolism; Bacteria - ultrastructure; Bacterial Physiological Phenomena; Biofilms; Carbohydrates - biosynthesis; Carbohydrates - chemistry; Corrosion; Gold - chemistry; Lectins - chemistry
• ISSN: 0555-1099
• DOI: 10.7868/S0555109913050103

The carbohydrate components of biofilms of corrosion-aggressive bacteria were studied by transmisstion electron microscopy using lectins labeled with colloidal gold. N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, and neutral carbohydrates D-glucose and D-mannose were found within the exopolymeric matrix. Lectins with equal carbohydrate specificity demonstrated different degrees of interaction with the carbohydrate components of bacterial biofilms. To identify N-acetyl-D-galactosamine in biofilms of Desulfovibrio sp. 10 and Bacillus subtilis 36, the LBA lectin appeared to be most specific; in the case of N-acetyl-D-glucosamine in biofilms of B. subtilis 36 and Pseudomonas aeruginosa 27, the WGA lectin. During visualization of neutral carbohydrates in the studied cultures, the PSA lectin was most specific. We have shown that lectins labeled with colloidal gold could be used as an express method for the identification and localization of carbohydrates in glycopolymers of the biofilm exopolymeric matrix.