A gas chromatography–mass spectrometry method for the measurement of fatty acid ω and ω−1 hydroxylation kinetics by CYP4A1 using an artificial membrane system
A gas chromatography–mass spectrometry assay method for the analysis of lauric, myristic, and palmitic acids and their ω and ω −1 hydroxylated metabolites from in vitro incubations of cytochrome P450 CYP4A1, involving solid-phase extraction and trimethysilyl derivatization, was developed. The assay...
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| Published in: | Analytical biochemistry Vol. 325; no. 2; pp. 354 - 363 |
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| Format: | Journal Article |
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15-02-2004
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| Abstract | A gas chromatography–mass spectrometry assay method for the analysis of lauric, myristic, and palmitic acids and their
ω and
ω
−1 hydroxylated metabolites from in vitro incubations of cytochrome P450 CYP4A1, involving solid-phase extraction and trimethysilyl derivatization, was developed. The assay was linear, precise, and accurate over the range 0.5 to 50
μM for all the analytes. It has the advantages of a more rapid analysis time, an improved sensitivity, and a wider range of analytes compared with other methods. An artificial membrane system was optimized for application to purified CYP4A1 enzyme by investigating the molar ratios of cytochrome
b
5 and cytochrome P450 reductase present in the incubation mixture. Using this method, the kinetics of
ω and
ω
−1 oxidation of lauric, myristic, and palmitic acids by CYP4A enzymes were measured and compared in rat liver microsomes and an artificial membrane system. |
|---|---|
| AbstractList | A gas chromatography–mass spectrometry assay method for the analysis of lauric, myristic, and palmitic acids and their
ω and
ω
−1 hydroxylated metabolites from in vitro incubations of cytochrome P450 CYP4A1, involving solid-phase extraction and trimethysilyl derivatization, was developed. The assay was linear, precise, and accurate over the range 0.5 to 50
μM for all the analytes. It has the advantages of a more rapid analysis time, an improved sensitivity, and a wider range of analytes compared with other methods. An artificial membrane system was optimized for application to purified CYP4A1 enzyme by investigating the molar ratios of cytochrome
b
5 and cytochrome P450 reductase present in the incubation mixture. Using this method, the kinetics of
ω and
ω
−1 oxidation of lauric, myristic, and palmitic acids by CYP4A enzymes were measured and compared in rat liver microsomes and an artificial membrane system. |
| Author | Holmes, Victoria E Shaw, P.Nicholas Bruce, Mary Barrett, David A Bell, David R Qi, Fan Ming |
| Author_xml | – sequence: 1 givenname: Victoria E surname: Holmes fullname: Holmes, Victoria E organization: Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK – sequence: 2 givenname: Mary surname: Bruce fullname: Bruce, Mary organization: Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK – sequence: 3 givenname: P.Nicholas surname: Shaw fullname: Shaw, P.Nicholas organization: Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK – sequence: 4 givenname: David R surname: Bell fullname: Bell, David R organization: Molecular Toxicology Group, School of Biology, University of Nottingham, Nottingham NG7 2RD, UK – sequence: 5 givenname: Fan Ming surname: Qi fullname: Qi, Fan Ming organization: Molecular Toxicology Group, School of Biology, University of Nottingham, Nottingham NG7 2RD, UK – sequence: 6 givenname: David A surname: Barrett fullname: Barrett, David A email: david.barrett@nottingham.ac.uk organization: Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK |
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| Cites_doi | 10.1016/0166-445X(96)00014-8 10.1016/S0022-2275(20)42618-5 10.1042/bj2750247 10.1016/S0003-2670(02)00206-4 10.1006/abbi.1995.1149 10.1177/096032719401300201 10.1093/oxfordjournals.jbchem.a123235 10.1016/0076-6879(91)06112-G 10.1093/oxfordjournals.jbchem.a124339 10.1016/0003-9861(92)90714-8 10.1093/oxfordjournals.jbchem.a124006 10.1006/abbi.1999.1352 10.1016/S0021-9258(18)37333-2 10.1007/s11745-997-0084-2 10.1007/s11745-997-0130-0 10.1111/j.1432-1033.1984.tb07999.x 10.1016/S0378-4347(98)00244-8 10.1016/S0003-2670(02)00467-1 10.1021/bi972458s 10.1042/bj2030161 10.1016/S0021-9258(19)75857-8 10.1016/S0306-3623(96)00246-7 10.1016/0003-2697(76)90527-3 10.1016/S0021-9258(19)61465-1 10.1016/0378-4347(91)80581-V 10.1006/abbi.1999.1504 |
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| Keywords | GC–MS CYP4A1 Artificial membrane Kinetics Hydroxylation Fatty acids |
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| Title | A gas chromatography–mass spectrometry method for the measurement of fatty acid ω and ω−1 hydroxylation kinetics by CYP4A1 using an artificial membrane system |
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