Screening of spiro-substituted thiopyrano[2,3-d]thiazoles for their cytotoxic action on tumor cells

• Published in: Biopolimery i kletka Vol. 33; no. 4; pp. 282 - 290
• Main Authors: Zelisko, N. I., Finiuk, N. S., Shvets, V. M., Medvid, Yu. O., Stoika, R. S., Lesyk, R. B.
• Format: Journal Article
• Language: English
• Published: Kiev Natsional'na Akademiya Nauk Ukrainy - National Academy of Sciences of Ukraine 2017
• Subjects: Adenocarcinoma; Breast; Cytotoxicity; Leukemia; Lymphocytes T; Mammalian cells; Melanoma; Non-small cell lung carcinoma; Ovarian carcinoma; Statistical analysis; Thiazoles; Tumor cell lines; Tumor cells
• ISSN: 0233-7657; 1993-6842
• DOI: 10.7124/bc.00095A

Aim. To evaluate the in vitro cytotoxicity of novel spiro-substituted thiopyrano[2,3-d]thiazoles towards tumor cells of different tissue origin. Methods. Organic synthesis; spectral methods; MTT test, statistical analysis. Results. In vitro screening of the cytotoxic activity of the 5’-carboxy-7’-aryl-1-aryl-3’,7’-dihydro-2H,2’H,5H-spiro[pyrolidin-3,6’-thiopyrano[2,3-d]thiazol]-2,2’,5-triones and N-(4-chlorophenyl)-2-[1-(4-chlorophenyl)-2,5-dioxopyrrolidin-3-ylidene]-acetamide was performed using various cancer cell lines (Jurkat human acute T-cell leukemia cell line, MCF-7 human breast adenocarcinoma cell line, Skov3 human ovarian carcinoma cells line, SK-Mel-28 human melanoma cells line, and SW-1573 human non-small-cell lung cancer cell line). The tested compounds possessed different cytotoxic action towards the studied tumor cells. Leukemia cells appeared to be more sensitive for the studied derivatives. The cytotoxic effect of the compound 2 towards Jurkat cells was shown to be dose- and time-dependent (3, 6, 24, 48 and 72 h). This compound demonstrated the cytotoxic action towards Jurkat cells as soon as in 6 h after its addition to the cultured cells (IC50 = 66 μM), and its toxicity towards these cells was more prominent after 24 h treatment (IC50= 40 μM). Conclusions. The panel of thiopyrano[2,3-d]thiazole derivatives was synthesized and screened for their cytotoxic activity in vitro towards tumor cells of different tissue origin. The compound 2 was found to be the most active agent with selectivity for the leukemia cells. This compound inhibits growth of the human acute T-cell leukemia cells of Jurkat line (IC50 = 33.5 μM) and possesses relatively low toxicity towards the pseudo-normal mammalian cells.