Nuclear heterogeneity and proliferation activity of human adipose derived MSC-like cells

• Published in: Cell and tissue biology Vol. 4; no. 5; pp. 452 - 456
• Main Authors: Lavrov, A. V., Smirnikhina, S. A.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01-10-2010
• Subjects: Biomedical and Life Sciences; Cell Biology; Life Sciences; mesenchymal stem-cell morphology; mesenchymal stem cell; adipose-derived stem cell; heterogeneity of mesenchymal stem cells
• ISSN: 1990-519X; 1990-5203
• DOI: 10.1134/S1990519X1005007X

Adipose tissue (AT) is an easily available source of mesenchymal-like stem cells (MSCs) that are appropriate for applications in regenerative medicine. There is conflicting evidence on the morphology of AT-derived stem cells (ADSCs). Here, we described the morphology and proliferation activity of human ADSCs. The cells were plated at a density of 10 cells/sm
2
and cultivated for 1 month. Twenty-one colonies were grown. In nine out of 17 analyzed colonies, few atypical cells (large nuclei and cytoplasm) were found. ANOVA demonstrated that colonies also differed (
p
= 0.0025) in the size and diameter of cell nuclei. The size of nuclei and logarithm of cell density were correlated in the reverse proportion (−0.7;
p
= 0.002). Thus, a culture obtained from the stromal vascular fraction (SVF) is heterogeneous and composed of two types of cells, i.e., highly proliferative and large, low proliferative cells. These cells are typical of the MSC and ADSC cultures described in literature. The cell heterogeneity observed in some colonies probably resulted from variations in the cell-cycle phases.