AdtsRGene-Disrupted Mutant ofBrevibacterium lactofermentumRequires Fatty Acids for Growth and Efficiently Produces L-Glutamate in the Presence of an Excess of Biotin

• Published in: Biochemical and biophysical research communications Vol. 234; no. 1; pp. 157 - 161
• Main Authors: Kimura, Eiichiro, Abe, Chizu, Kawahara, Yoshio, Nakamatsu, Tsuyoshi, Tokuda, Hajime
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01-05-1997
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1997.6613

AdtsRgene encoding a homolog of the β subunit of some biotin-containing enzymes suppresses a detergent-sensitive mutation ofBrevibacterium lactofermentum(E. Kimuraet al.,1996,Biosci. Biotech. Biochem.60, 1565–1570), which has been used for the fermentative production of L-glutamate. When thedtsRgene was disrupted, the organism exhibited strict fatty acid auxotrophy; oleate or oleate ester, but not palmitate ester or stearate ester, supported the growth of the ΔdtsRmutant. Immunoblotting with an anti-DtsR antibody revealed that no intact DtsR was present in the cytosol of the ΔdtsRmutant. In the presence of an excess of biotin, the wild type strain did not produce L-glutamate whereas the ΔdtsRmutant efficiently produced it. The mechanism underlying the efficient production of L-glutamate by the ΔdtsRmutant is discussed as to the possible role ofdtsRin fatty acid metabolism.