Candidate Genes for Brown Fiber in Cotton Revealed Among the R2R3-Myb and bHLH-Myc Gene Families

Naturally colored cotton varieties attract investigators due to their eco-friendly and hypoallergenic properties. Genetic mechanisms, determining control of pigment biosynthesis pathway in cotton fiber, are being intensively studied nowadays. However, the knowledge, explaining control of proanthocya...

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Published in:	Journal of natural fibers Vol. 21; no. 1
Main Authors:	Mikhailova, Aleksandra S., Shvachko, Natalia A., Podolnaya, Larisa P., Brutch, Nina B., Khlestkina, Elena K.
Format:	Journal Article
Language:	English
Published:	Taylor & Francis Group 31-12-2024
Subjects:	bHLH-Myc flavonoids MBW complex naturally colored cotton proanthocyanidins R2R3-Myb
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Abstract	Naturally colored cotton varieties attract investigators due to their eco-friendly and hypoallergenic properties. Genetic mechanisms, determining control of pigment biosynthesis pathway in cotton fiber, are being intensively studied nowadays. However, the knowledge, explaining control of proanthocyanidins accumulation in cotton fiber by the MBW complex (R2R3-Myb, bHLH-Myc and WD40), is insufficient. In this connection, we focused on the regulatory genes from a number of the most likely ones involved in proanthocyanidins biosynthesis. We used the Sanger sequencing approach to reveal the allelic diversity of the R2R3-Myb and bHLH-Myc genes among contrast genotype accessions including various fiber shades and hypocotyl color. Having used the number of bioinformatics tools, we concluded that the replacement of conserved tryptophan residue abolished the DNA-binding activity of the GhMYB10-A transcription factor and this, probably, can be associated with the light brown fiber phenotype. Deeper studies are required to definitely establish the GhMYB10-A function. However, our achievements can be practically applied in marker-assisted breeding of cotton varieties with naturally colored fiber.
AbstractList	Naturally colored cotton varieties attract investigators due to their eco-friendly and hypoallergenic properties. Genetic mechanisms, determining control of pigment biosynthesis pathway in cotton fiber, are being intensively studied nowadays. However, the knowledge, explaining control of proanthocyanidins accumulation in cotton fiber by the MBW complex (R2R3-Myb, bHLH-Myc and WD40), is insufficient. In this connection, we focused on the regulatory genes from a number of the most likely ones involved in proanthocyanidins biosynthesis. We used the Sanger sequencing approach to reveal the allelic diversity of the R2R3-Myb and bHLH-Myc genes among contrast genotype accessions including various fiber shades and hypocotyl color. Having used the number of bioinformatics tools, we concluded that the replacement of conserved tryptophan residue abolished the DNA-binding activity of the GhMYB10-A transcription factor and this, probably, can be associated with the light brown fiber phenotype. Deeper studies are required to definitely establish the GhMYB10-A function. However, our achievements can be practically applied in marker-assisted breeding of cotton varieties with naturally colored fiber.
Author	Podolnaya, Larisa P. Shvachko, Natalia A. Khlestkina, Elena K. Brutch, Nina B. Mikhailova, Aleksandra S.
Author_xml	– sequence: 1 givenname: Aleksandra S. orcidid: 0000-0003-4565-1539 surname: Mikhailova fullname: Mikhailova, Aleksandra S. organization: Laboratory of Postgenomic Investigations, Federal Research Center N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), St.-Petersburg, Russia – sequence: 2 givenname: Natalia A. surname: Shvachko fullname: Shvachko, Natalia A. organization: Laboratory of Postgenomic Investigations, Federal Research Center N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), St.-Petersburg, Russia – sequence: 3 givenname: Larisa P. surname: Podolnaya fullname: Podolnaya, Larisa P. organization: Genetic Resources of Oil and Fiber Crops, Federal Research Center N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), St.-Petersburg, Russia – sequence: 4 givenname: Nina B. orcidid: 0000-0003-2253-6263 surname: Brutch fullname: Brutch, Nina B. organization: Genetic Resources of Oil and Fiber Crops, Federal Research Center N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), St.-Petersburg, Russia – sequence: 5 givenname: Elena K. surname: Khlestkina fullname: Khlestkina, Elena K. organization: Federal Research Center N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), Krasnodar Region, Sochi, Russia
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SubjectTerms	bHLH-Myc flavonoids MBW complex naturally colored cotton proanthocyanidins R2R3-Myb
Title	Candidate Genes for Brown Fiber in Cotton Revealed Among the R2R3-Myb and bHLH-Myc Gene Families
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