Isolated and Associated Use of the Nematophagous Fungi Pochonia chlamydosporia and Duddingtonia flagrans to Control Taenia saginata Eggs

• Published in: Parasitologia (Basel, Switzerland) Vol. 4; no. 3; pp. 238 - 245
• Main Authors: Ferreira, Thaís Schmidt, Ferraz, Carolina Magri, Santos, Pedro Henrique Dutra, Soares, Filippe Elias Freitas, Segantine, Vinicius Bastos Salles, Vilela, Vinícius Longo Ribeiro, Araújo, Jackson Victor, Braga, Fabio Ribeiro
• Format: Journal Article
• Language: English
• Published: MDPI AG 01-09-2024
• Subjects: biological control; biotechnology; helminths; isolate AC001; isolate VC4; one health
• ISSN: 2673-6772; 2673-6772
• DOI: 10.3390/parasitologia4030020

The aim of this study was to evaluate the isolated and associated use of the nematophagous fungi Pochonia chlamydosporia (isolate VC4) as an ovicide and Duddingtonia flagrans (isolate AC001) as a predator in the control of Taenia saginata eggs. Viable T. saginata eggs were obtained by dissecting mature proglottids from a specimen. For the experimental assay, four groups were formed in microtubes, as follows: (G1—T. saginata eggs/control); (G2—100 T. saginata eggs + VC4); (G3—100 T. saginata eggs + AC001); (G4—100 T. saginata eggs + VC4 + AC001). All the groups were stored in a B.O.D. incubation chamber at a temperature of 27 °C for 15 days and then the contents of the microtubes were analyzed using an optical microscope with a 40x objective. At the end of the assay the treated groups (G2 to G4) showed ovicidal activity (destruction of eggs) compared to the control group (G1). The highest ovicidal percentage was observed in group G2 (eggs + VC4), with 43.3%. In groups G3 and G4 (combination of fungal isolates), the ovicidal percentages were 25.7% and 25.6%, respectively. The results of this study shed light on a new possibility for the combined use of different species of nematophagous fungi, which could be used in the future for environmental biological control of T. saginata eggs.