Anti-STAT6 CTL activity in Stat6−/− mice A cautionary tale
The generation of germline gene mutations in mice has been an invaluable tool for experimental biology. However, studying immune responses that develop in the absence of a specific protein that could alter thymic selection complicates experimental interpretations. We observed that CD8 + T cells from...
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| Published in: | JAK-STAT Vol. 2; no. 2; p. e24554 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
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Taylor & Francis
01-04-2013
Landes Bioscience |
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| Abstract | The generation of germline gene mutations in mice has been an invaluable tool for experimental biology. However, studying immune responses that develop in the absence of a specific protein that could alter thymic selection complicates experimental interpretations. We observed that CD8
+
T cells from Stat6
−/−
mice displayed "autoreactivity" to STAT6-expressing cells, associated with specific STAT6 peptides binding to MHC class I molecules. These results suggest caution in interpreting experiments where STAT6-expressing cells are transferred into Stat6
−/−
mice, or where adoptive transfer of Stat6
−/−
lymphocytes is performed. Our results further highlight additional considerations when studying immune responses involving cell transfer into gene-deficient mice. |
|---|---|
| AbstractList | The generation of germline gene mutations in mice has been an invaluable tool for experimental biology. However, studying immune responses that develop in the absence of a specific protein that could alter thymic selection complicates experimental interpretations. We observed that CD8(+) T cells from Stat6 (-/-) mice displayed "autoreactivity" to STAT6-expressing cells, associated with specific STAT6 peptides binding to MHC class I molecules. These results suggest caution in interpreting experiments where STAT6-expressing cells are transferred into Stat6 (-/-) mice, or where adoptive transfer of Stat6 (-/-) lymphocytes is performed. Our results further highlight additional considerations when studying immune responses involving cell transfer into gene-deficient mice. The generation of germline gene mutations in mice has been an invaluable tool for experimental biology. However, studying immune responses that develop in the absence of a specific protein that could alter thymic selection complicates experimental interpretations. We observed that CD8 + T cells from Stat6 −/− mice displayed "autoreactivity" to STAT6-expressing cells, associated with specific STAT6 peptides binding to MHC class I molecules. These results suggest caution in interpreting experiments where STAT6-expressing cells are transferred into Stat6 −/− mice, or where adoptive transfer of Stat6 −/− lymphocytes is performed. Our results further highlight additional considerations when studying immune responses involving cell transfer into gene-deficient mice. The generation of germline gene mutations in mice has been an invaluable tool for experimental biology. However, studying immune responses that develop in the absence of a specific protein that could alter thymic selection complicates experimental interpretations. We observed that CD8 + T cells from Stat6 −/− mice displayed “autoreactivity” to STAT6-expressing cells, associated with specific STAT6 peptides binding to MHC class I molecules. These results suggest caution in interpreting experiments where STAT6-expressing cells are transferred into Stat6 −/− mice, or where adoptive transfer of Stat6 −/− lymphocytes is performed. Our results further highlight additional considerations when studying immune responses involving cell transfer into gene-deficient mice. |
| Author | Aldrich, Carla J. Cundiff, Judy K. Kaplan, Mark H. Smith, Jill Stader |
| Author_xml | – sequence: 1 givenname: Mark H. surname: Kaplan fullname: Kaplan, Mark H. email: mkaplan2@iupui.edu – sequence: 2 givenname: Judy K. surname: Cundiff fullname: Cundiff, Judy K. – sequence: 3 givenname: Jill Stader surname: Smith fullname: Smith, Jill Stader – sequence: 4 givenname: Carla J. surname: Aldrich fullname: Aldrich, Carla J. |
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| Cites_doi | 10.4049/jimmunol.165.11.6015 10.1038/382174a0 10.4049/jimmunol.167.3.1683 10.1067/mai.2002.123531 10.1007/BF00364232 10.1016/S1074-7613(00)80439-2 10.1084/jem.191.6.995 10.4049/jimmunol.165.10.5580 10.4049/jimmunol.169.7.3744 10.1111/j.1365-2567.2008.02935.x 10.1172/JCI200112563 10.1016/j.jneuroim.2008.11.003 10.1073/pnas.0506981103 10.4049/jimmunol.134.5.3218 10.1007/s12026-011-8205-2 10.1007/BF00375374 10.4049/jimmunol.165.11.6024 10.1038/380630a0 10.4049/jimmunol.170.4.2014 10.4049/jimmunol.131.5.2147 10.1038/380627a0 10.4161/jkst.19086 |
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| Keywords | cytotoxic T cell MHC class I autoreactivity STAT6 chromium release |
| Language | English |
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| SubjectTerms | autoreactivity chromium release cytotoxic T cell MHC class I Research Paper STAT6 |
| Subtitle | A cautionary tale |
| Title | Anti-STAT6 CTL activity in Stat6−/− mice |
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