Development of the CE-C4D method for determination of organic acids in infants faeces using an in-house built instrument
[Display omitted] •SCFAs, crucial for health, result from bacterial fermentation of dietary fibres.•CE-C4D method for SCFAs analysis in infants' faeces was developed.•Polybrene dynamic capillary coating enables the separation of SCFAs isomers.•Analysis of faeces gives insight into microbial met...
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| Published in: | Microchemical journal Vol. 199; p. 110203 |
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| Main Authors: | , , , , , , , |
| Format: | Journal Article |
| Language: | English |
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Elsevier B.V
01-04-2024
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| Abstract | [Display omitted]
•SCFAs, crucial for health, result from bacterial fermentation of dietary fibres.•CE-C4D method for SCFAs analysis in infants' faeces was developed.•Polybrene dynamic capillary coating enables the separation of SCFAs isomers.•Analysis of faeces gives insight into microbial metabolism in infants' intestines.
Short-chain fatty acids (SCFAs) are a major group of products produced during colonic bacterial fermentation of nondigestible carbohydrates demonstrating several important functions, e.g. regulating the immune system activity or the lipid metabolism. A detailed profile and concentration characterization of SCFAs in infant faeces is essential for expanding knowledge regarding the role of SCFAs in infant physiology and pathophysiology. This research presents the development and validation of a capillary electrophoresis method with a contactless capacitively coupled conductivity detector (CE-C4D) for the analysis of SCFAs and succinic and lactic acids in infant faeces. The study focused on optimizing the background electrolyte, mainly dynamic capillary coating with polybrene, and utilizing cyclodextrins to achieve efficient separation. Validation parameters, such as linearity (0.993 – 0.998), limits of detection and quantification (LOD: 0.10 – 0.89 μg/mL; LOQ: 0.35 – 2.97 μg/mL), intra- and inter-day precision (1.71 – 5.69 % and 1.11 – 12.5 %, respectively), injection repeatability (0.91 – 5.70 %), trueness (-12.8 – 3.80 %), and matrix effect (78.8 – 108.6 %), were assessed and the method was found suitable for the purpose. The demonstrated method was successfully applied to determine nine organic acids in faecal samples. |
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| AbstractList | [Display omitted]
•SCFAs, crucial for health, result from bacterial fermentation of dietary fibres.•CE-C4D method for SCFAs analysis in infants' faeces was developed.•Polybrene dynamic capillary coating enables the separation of SCFAs isomers.•Analysis of faeces gives insight into microbial metabolism in infants' intestines.
Short-chain fatty acids (SCFAs) are a major group of products produced during colonic bacterial fermentation of nondigestible carbohydrates demonstrating several important functions, e.g. regulating the immune system activity or the lipid metabolism. A detailed profile and concentration characterization of SCFAs in infant faeces is essential for expanding knowledge regarding the role of SCFAs in infant physiology and pathophysiology. This research presents the development and validation of a capillary electrophoresis method with a contactless capacitively coupled conductivity detector (CE-C4D) for the analysis of SCFAs and succinic and lactic acids in infant faeces. The study focused on optimizing the background electrolyte, mainly dynamic capillary coating with polybrene, and utilizing cyclodextrins to achieve efficient separation. Validation parameters, such as linearity (0.993 – 0.998), limits of detection and quantification (LOD: 0.10 – 0.89 μg/mL; LOQ: 0.35 – 2.97 μg/mL), intra- and inter-day precision (1.71 – 5.69 % and 1.11 – 12.5 %, respectively), injection repeatability (0.91 – 5.70 %), trueness (-12.8 – 3.80 %), and matrix effect (78.8 – 108.6 %), were assessed and the method was found suitable for the purpose. The demonstrated method was successfully applied to determine nine organic acids in faecal samples. |
| ArticleNumber | 110203 |
| Author | Dobrowolska-Iwanek, Justyna Staśkiewicz, Magdalena Woźniakiewicz, Michał Pluta, Joanna Rusin, Marcelina Lauterbach, Ryszard Huras, Hubert Woźniakiewicz, Aneta |
| Author_xml | – sequence: 1 givenname: Marcelina orcidid: 0000-0003-2668-6664 surname: Rusin fullname: Rusin, Marcelina organization: Jagiellonian University, Doctoral School of Exact and Natural Sciences, prof. St. Łojasiewicza 11, 30-348 Kraków, Poland – sequence: 2 givenname: Joanna orcidid: 0009-0009-2217-9279 surname: Pluta fullname: Pluta, Joanna organization: Jagiellonian University, Doctoral School of Exact and Natural Sciences, prof. St. Łojasiewicza 11, 30-348 Kraków, Poland – sequence: 3 givenname: Aneta surname: Woźniakiewicz fullname: Woźniakiewicz, Aneta organization: Jagiellonian University, Faculty of Chemistry, Department of Analytical Chemistry, Gronostajowa 2, 30-387 Kraków, Poland – sequence: 4 givenname: Justyna surname: Dobrowolska-Iwanek fullname: Dobrowolska-Iwanek, Justyna organization: Jagiellonian University Medical College, Faculty of Pharmacy, Department of Food Chemistry and Nutrition, Medyczna 9, 31-008 Kraków, Poland – sequence: 5 givenname: Hubert surname: Huras fullname: Huras, Hubert organization: Department of Obstetrics and Perinatology, Jagiellonian University Hospital, Kopernika 23, 31-501 Kraków, Poland – sequence: 6 givenname: Magdalena surname: Staśkiewicz fullname: Staśkiewicz, Magdalena organization: Department of Obstetrics and Perinatology, Jagiellonian University Hospital, Kopernika 23, 31-501 Kraków, Poland – sequence: 7 givenname: Ryszard surname: Lauterbach fullname: Lauterbach, Ryszard organization: Department of Neonatology, Jagiellonian University Hospital, Kopernika 23, 31-501 Kraków, Poland – sequence: 8 givenname: Michał orcidid: 0000-0002-1348-2884 surname: Woźniakiewicz fullname: Woźniakiewicz, Michał email: michal.wozniakiewicz@uj.edu.pl organization: Jagiellonian University, Faculty of Chemistry, Department of Analytical Chemistry, Gronostajowa 2, 30-387 Kraków, Poland |
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| Cites_doi | 10.1039/C9AY01202H 10.1002/elps.201900319 10.1016/j.microc.2020.104671 10.1016/S0021-9673(04)01201-4 10.1111/1541-4337.12148 10.1016/j.cca.2022.05.005 10.1152/physrev.2001.81.3.1031 10.1016/j.jchromb.2020.121972 10.1016/j.ab.2017.03.007 10.1194/jlr.R036012 10.3390/nu15020367 10.1016/j.jpba.2016.02.042 10.1016/j.gpb.2018.10.002 10.1093/jat/bkt054 10.1016/S0165-9936(01)00067-X 10.1016/j.talanta.2018.12.049 10.1016/j.jpba.2007.10.026 10.1016/j.chroma.2018.03.026 10.1080/10408340500451957 10.1111/1462-2920.13589 10.1021/acs.analchem.9b01872 10.3390/jcm10204701 10.1002/jssc.200700306 10.1016/j.aca.2022.340161 10.1002/elps.200600654 10.1016/j.jchromb.2009.01.039 10.1016/j.jpba.2020.113658 10.1016/j.aca.2007.11.045 |
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