Osteogenic differentiation by MC3T3-E1 pre-osteoblasts is enhanced more on wet-chemically surface-modified 3D-printed poly-e-caprolactone scaffolds than on plasma-assisted modified scaffolds

• Published in: Applied surface science Vol. 671; p. 160750
• Main Authors: Seddiqi, Hadi, Abbasi-Ravasjani, Sonia, Moghaddaszadeh, Ali, Ehsan Ghiasvand, Mohammad, Yahyazadeh, Amin, Yahyazadeh, Ehsan, Hajipour-Verdom, Behnam, Jin, Jianfeng, Klein-Nulend, Jenneke
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 30-10-2024
• Subjects: 3D-printed scaffold; bone tissue engineering; Finite element modeling; Poly-є-caprolactone; Pre-osteoblast; Surface functionalization; Surface functionalization; Poly-є-caprolactone; Finite element modeling; bone tissue engineering; 3D-printed scaffold; Pre-osteoblast
• ISSN: 0169-4332; 1873-5584
• DOI: 10.1016/j.apsusc.2024.160750

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•Surface-functionalization by wet-chemical treatment enhances osteogenic activity.•Carboxyl-functionalization by wet-chemical treatment is most promising.•Wet-chemical treatment modulates biophysicochemical and biomechanical properties.•Wet-chemical treatment improves scaffold hydrophilicity, roughness, and bioactivity.•Surface-modified scaffolds do not exceed yield stress for 2% compression strain.
3D-Printed poly-є-caprolactone (PCL)-scaffolds are safe for cell support, but their hydrophobicity, bioinertness, and smooth surface limits bioactive/biomimetic performance. This can be overcome by providing biophysicochemical and biomechanical signals using amine (NH2) or carboxyl (COOH)-surface functionalization via wet-chemical treatment or plasma-assisted modification, but the alteration extent and hence bone-cell activity is unknown. We aimed to investigate the influence of NH2 or COOH-surface functionalization via wet-chemical treatment or plasma-assisted modification on biophysicochemical and biomechanical properties of 3D-printed PCL-scaffolds and osteogenic activity. In PCL/NH2 and PCL/COOH scaffolds, only wet-chemical treatment increased void size and surface-roughness, but both treatments reduced water contact angle. Wet-chemical treatment decreased, but plasma-assisted modification increased the elastic modulus. Finite-element modeling revealed that in all scaffolds, maximum von Mises stress under 2% compression-strain did not surpass yield stress for bulk material, indicating excellent mechanical stability and fracture resistance. Wet-chemical treatment and plasma-assisted modification increased pre-osteoblast proliferation, alkaline phosphatase activity, and mineral deposition. Wet-chemical treatment increased Fgf2 mRNA, while both treatments increased Cox2 mRNA. Only wet-chemical treatment increased collagen production. In conclusion, NH2 and COOH-surface functionalization of 3D-printed PCL-scaffolds via wet-chemical treatment was superior to plasma-assisted modification in enhancing osteogenic activity, and is therefore more promising for in vivo bone formation.