Paenibacillus suaedae sp. nov. and Paenibacillus violae sp. nov., isolated from the roots of Suaeda japonica Makino and Viola mandshurica W. Becker with plant growth-promoting potential

• Published in: International journal of systematic and evolutionary microbiology Vol. 74; no. 10
• Main Authors: Kim, Inhyup, Park, Sunho, Jung, Yonghee, Woo, Haejin, Lee, Hyunji, Yook, Subin, Seo, Taegun
• Format: Journal Article
• Language: English
• Published: England 01-10-2024
• Subjects: Bacterial Typing Techniques; Base Composition; Chenopodiaceae - microbiology; DNA, Bacterial - genetics; Fatty Acids - analysis; Nucleic Acid Hybridization; Paenibacillus - classification; Paenibacillus - genetics; Paenibacillus - isolation & purification; Phylogeny; Plant Roots - microbiology; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - analysis; Viola mandshurica; Paenibacillus; Suaeda japonica Makino; novel species; genome analysis; rhizosphere
• ISSN: 1466-5034
• DOI: 10.1099/ijsem.0.006542

Two novel Gram-positive strains, chi10
and PFR10
, were isolated from the roots of
Makino and
W. Becker, respectively. The strains are facultatively aerobic, rod-shaped, motile via peritrichous flagella and endospore-forming. Strains chi10
and PFR10
showed the highest 16S rRNA gene sequence similarity to
DSM 29
(99.0%) and
LMG 31457
(99.3%). Furthermore, the phylogenetic and phylogenomic analyses demonstrated that strains chi10
and PFR10
were closely related to the genus
. The digital DNA-DNA hybridization (dDDH) values for strains used in the phylogenetic analysis and strain chi10
ranged from 19.6 to 28.4%, while for strain PFR10
, the values ranged from 19.8 to 53.5%, according to the Genome-to-Genome Distance Calculator 3.0, which were all less than 70%. Additionally, the dDDH value between strains chi10
and PFR10
was 22.0%, confirming that they are different species of the genus
. The average nucleotide identity between chi10
and PFR10
and other species of the genus
were 68.4-84.7% and 67.8-93.6%, respectively. In addition, the major polar lipids of chi10
and PFR10
consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acid profile of strain chi10
was iso-C
and anteiso-C
, whereas the major fatty acid profile of strain PFR10
was iso-C
, anteiso-C
and iso-C
. The sole respiratory quinone in strains chi10
and PFR10
was menaquinone-7. Phylogenomic, phenotypic and genome analyses strongly indicated that chi10
and PFR10
could be two novel
species for which the names
sp. nov. (type strain chi10
= KACC 23258
= TBRC 17803
) and
sp. nov. (type strain PFR10
= KACC 23263
= TBRC 17804
) are proposed, respectively.