Molecular detection of two virulence factors of Pseudomonas aeruginosa isolated from burn patients

• Published in: مجلة علوم ذي قار Vol. 7; no. 1; pp. 62 - 65
• Main Authors: al-Maliki, Haydar Khamis, Degaim, Zaynab Dakhil, Nasir, Ahmad A., Nassar, Ala Mithqal
• Format: Journal Article
• Language: English
• Published: Dhi-Qar, Iraq University of Thi-Qar, College of Science 19-06-2019; University of Thi-Qar
• Subjects: burn; exoS; P. aeruginosa; phzS genes; virulence factors
• ISSN: 1991-8690; 2709-0256
• DOI: 10.32792/utq/utjsci/v7i1.251

The present study aimed to identify two virulence genes in P. aeruginosa isolated from infected burn wound. Out of 70 burn swabs, 54 (77%) showed positive growth on enriched media which obtained from burn wound at Al-Mussan Teaching Hospital in Mussan province, Iraq. Only 13 isolates (35%) identified as P. aeruginosa, and those isolates examined using Polymerase Chain Reaction (PCR) for detection the exoS and phzS genes. The results recorded that 46% and 77% of isolates have exoS, phzS genes, respectively. We concluded a P. aeruginosa harbor diverse percentages of both genes as virulence factors, and those factors must be increased the pathogenicity of this pathogen.