Does a single oral administration of amiloride affect spontaneous arterial baroreflex sensitivity and blood pressure variability in healthy young adults?

• Published in: Journal of neurophysiology Vol. 132; no. 3; pp. 922 - 928
• Main Authors: Fernandes, Igor A, Stavres, Jon, Hamaoka, Takuto, Ojikutu, Qudus A, Sabino-Carvalho, Jeann L, Vianna, Lauro C, Luck, J Carter, Blaha, Cheryl, Cauffman, Aimee E, Dalton, Paul C, Herr, Michael D, Ruiz-Velasco, Victor, Carr, Zyad J, Janicki, Piotr K, Cui, Jian
• Format: Journal Article
• Language: English
• Published: United States 01-09-2024
• Subjects: Administration, Oral; Adult; Amiloride - administration & dosage; Amiloride - pharmacology; Baroreflex - drug effects; Baroreflex - physiology; Blood Pressure - drug effects; Blood Pressure - physiology; Epithelial Sodium Channel Blockers - administration & dosage; Epithelial Sodium Channel Blockers - pharmacology; Female; Heart Rate - drug effects; Humans; Male; Sympathetic Nervous System - drug effects; Sympathetic Nervous System - physiology; Young Adult; muscle sympathetic nerve activity; blood pressure; acid-sensing ion channels
• DOI: 10.1152/jn.00264.2024
• EISSN: 1522-1598
• ISSN: 0022-3077; 1522-1598

Preclinical models indicate that amiloride (AMD) reduces baroreflex sensitivity and perturbs homeostatic blood pressure (BP) regulation. However, it remains unclear whether these findings translate to humans. This study investigated whether oral administration of AMD reduces spontaneous cardiac and sympathetic baroreflex sensitivity and perturbs BP regulation in healthy young humans. Heart rate (HR; electrocardiography), beat-to-beat BP (photoplethysmography), and muscle sympathetic activity (MSNA, microneurography) were continuously measured in 10 young subjects (4 females) during rest across two randomized experimental visits:
) after 3 h of oral administration of placebo (PLA, 10 mg of methylcellulose within a gelatin capsule) and
) after 3 h of oral administration of AMD (10 mg). Visits were separated for at least 48 h. We calculated the standard deviation and other indices of BP variability. Spontaneous cardiac baroreflex was assessed via the sequence technique and cardiac autonomic modulation through time- and frequency-domain HR variability. The sensitivity (gain) of the sympathetic baroreflex was determined via weighted linear regression analysis between MSNA and diastolic BP. AMD did not affect HR, BP, and MSNA compared with PLA. Indexes of cardiac autonomic modulation (time- and frequency-domain HR variability) and BP variability were also unchanged after AMD ingestion. Likewise, AMD did not modify the gain of both spontaneous cardiac and sympathetic arterial baroreflex. A single oral dose of AMD does not affect spontaneous arterial baroreflex sensitivity and BP variability in healthy young adults.
Preclinical models indicate that amiloride (AMD), a nonselective antagonist of the acid-sensing ion channels (ASICs), impairs baroreflex sensitivity and perturbs blood pressure regulation. We translated these findings into humans, investigating the impact of acute oral ingestion of AMD on blood pressure variability and spontaneous cardiac and sympathetic baroreflex sensitivity in healthy young humans. In contrast to preclinical evidence, AMD does not impair spontaneous arterial baroreflex sensitivity and blood pressure variability in healthy young adults.