Mitochondrial dynamics regulate cell morphology in the developing cochlea

• Published in: Development (Cambridge) Vol. 151; no. 15
• Main Authors: O'Sullivan, James D B, Terry, Stephen, Scott, Claire A, Bullen, Anwen, Jagger, Daniel J, Mann, Zoë F
• Format: Journal Article
• Language: English
• Published: England 01-08-2024
• Subjects: Animals; Cell Differentiation; Cell Shape; Chick Embryo; Chickens; Cochlea - cytology; Cochlea - embryology; Cochlea - growth & development; Hair Cells, Auditory - cytology; Hair Cells, Auditory - metabolism; Mitochondria - metabolism; Mitochondrial Dynamics; Hearing; Mitofusin; Biogenesis; Metabolism; Chick
• ISSN: 1477-9129
• DOI: 10.1242/dev.202845

In multicellular tissues, the size and shape of cells are intricately linked with their physiological functions. In the vertebrate auditory organ, the neurosensory epithelium develops as a mosaic of sensory hair cells (HCs), and their glial-like supporting cells, which have distinct morphologies and functional properties at different frequency positions along its tonotopic long axis. In the chick cochlea, the basilar papilla (BP), proximal (high-frequency) HCs, are larger than their distal (low-frequency) counterparts, a morphological feature essential for sound perception. Mitochondrial dynamics, which constitute the equilibrium between fusion and fission, regulate differentiation and functional refinement across a variety of cell types. We investigate this as a potential mechanism for regulating the shape of developing HCs. Using live imaging in intact BP explants, we identify distinct remodelling of mitochondrial networks in proximal compared with distal HCs. Manipulating mitochondrial dynamics in developing HCs alters their normal morphology along the proximal-distal (tonotopic) axis. Inhibition of the mitochondrial fusion machinery decreased proximal HC surface area, whereas promotion of fusion increased the distal HC surface area. We identify mitochondrial dynamics as a key regulator of HC morphology in developing inner ear epithelia.