Рroblems of PCR diagnostics OF COVID-19
To date, an adequate and timely assessment of the number of cases is the basis of effective measures aimed at preventing the spread of COVID-19 infection. Real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard for confirming COVID-19. The purpose of the work: to...
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| Published in: | Zhurnal infektologii Vol. 14; no. 3; pp. 55 - 60 |
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| Main Authors: | , |
| Format: | Journal Article |
| Language: | English Russian |
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Journal Infectology
22-10-2022
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| Online Access: | Get full text |
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| Abstract | To date, an adequate and timely assessment of the number of cases is the basis of effective measures aimed at preventing the spread of COVID-19 infection. Real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard for confirming COVID-19.
The purpose
of the work: to analyze the experience of the city virological center of the S.P. Botkin Clinical Infectious Diseases Hospital (Botkin Hospital) for the examination for the presence of SARS-CoV-2 coronavirus by PCR in the period from 2020 to 2022.
Materials and methods
. The systematization of PCR studies on COVID-19 for the period 2020-2022 was carried out. A total of 221,901 people were examined, positive results were obtained in 55,372 (24.95%). Among the contingents of the examined patients, patients who underwent inpatient treatment at the Botkin Hospital.
Conclusions.
This study analyzed the possible causes of false-positive and false-negative PCR results. The correlation of the number of positive results with the dynamics of detection of new cases of COVID-19 in St. Petersburg during the 2020-2022 pandemic is shown. It has been established that the proportion of patients examined more than 3 times during the period of hospitalization remains significant. This fact requires the closest attention, given the high cost and laboriousness of PCR studies. |
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| AbstractList | To date, an adequate and timely assessment of the number of cases is the basis of effective measures aimed at preventing the spread of COVID-19 infection. Real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard for confirming COVID-19.The purpose of the work: to analyze the experience of the city virological center of the S.P. Botkin Clinical Infectious Diseases Hospital (Botkin Hospital) for the examination for the presence of SARS-CoV-2 coronavirus by PCR in the period from 2020 to 2022.Materials and methods. The systematization of PCR studies on COVID-19 for the period 2020-2022 was carried out. A total of 221,901 people were examined, positive results were obtained in 55,372 (24.95%). Among the contingents of the examined patients, patients who underwent inpatient treatment at the Botkin Hospital.Conclusions. This study analyzed the possible causes of false-positive and false-negative PCR results. The correlation of the number of positive results with the dynamics of detection of new cases of COVID-19 in St. Petersburg during the 2020-2022 pandemic is shown. It has been established that the proportion of patients examined more than 3 times during the period of hospitalization remains significant. This fact requires the closest attention, given the high cost and laboriousness of PCR studies. To date, an adequate and timely assessment of the number of cases is the basis of effective measures aimed at preventing the spread of COVID-19 infection. Real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard for confirming COVID-19. The purpose of the work: to analyze the experience of the city virological center of the S.P. Botkin Clinical Infectious Diseases Hospital (Botkin Hospital) for the examination for the presence of SARS-CoV-2 coronavirus by PCR in the period from 2020 to 2022. Materials and methods . The systematization of PCR studies on COVID-19 for the period 2020-2022 was carried out. A total of 221,901 people were examined, positive results were obtained in 55,372 (24.95%). Among the contingents of the examined patients, patients who underwent inpatient treatment at the Botkin Hospital. Conclusions. This study analyzed the possible causes of false-positive and false-negative PCR results. The correlation of the number of positive results with the dynamics of detection of new cases of COVID-19 in St. Petersburg during the 2020-2022 pandemic is shown. It has been established that the proportion of patients examined more than 3 times during the period of hospitalization remains significant. This fact requires the closest attention, given the high cost and laboriousness of PCR studies. |
| Author | Belopolskaya, M. А. Grigorieva, T. D. |
| Author_xml | – sequence: 1 givenname: T. D. surname: Grigorieva fullname: Grigorieva, T. D. organization: Clinical Infectious Diseases Hospital named after S.P. Botkin – sequence: 2 givenname: M. А. surname: Belopolskaya fullname: Belopolskaya, M. А. organization: Clinical Infectious Diseases Hospital named after S.P. Botkin; Institute of Experimental Medicine |
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| Cites_doi | 10.1080/14737159.2020.1757437 10.1016/j.cell.2020.04.011 10.1136/bmj.m1808 10.2807/1560-7917.ES.2020.25.5.2000062 10.1001/jama.2020.3204 10.1001/jama.2020.3786 10.1016/j.pathol.2020.08.002 10.1056/NEJMoa2001316 10.1136/bmj.m1443 10.7861/clinmed.2020-0839 10.1016/S2213-2600(20)30453-7 10.1186/s40249-020-00646-x 10.1016/j.bbadis.2020.165878 10.1080/22221751.2020.1732837 10.1007/s10930-020-09901-4 10.1016/j.ijid.2020.03.062 10.2807/1560-7917.ES.2020.25.15.2000125 10.1016/S1473-3099(20)30200-0 10.1056/NEJMc2001737 |
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