Gastroprotective and ulcer healing effects of Nauclea pobeguinii (Rubiaceae) on experimentally induced gastric ulcers in male Wistar rats

• Published in: Journal of complementary & integrative medicine Vol. 20; no. 4; p. 729
• Main Authors: Madjo, Karelle Kouam Yacine, Mbiantcha, Marius, Fagni, Linda Zenab Njoya, Matah Marthe, Vanessa Mba, Ngoufack, Elvira Azanze, Tsafack, Gonzal Eric, Djuichou Nguemnang, Stephanie Flore, Adjouzem, Carine Flore, Ateufack, Gilbert
• Format: Journal Article
• Language: English
• Published: Germany 01-12-2023
• Subjects: Acetylcholine - adverse effects; Animals; Anti-Ulcer Agents - pharmacology; Anti-Ulcer Agents - therapeutic use; Ethanol - pharmacology; Gastric Mucosa; Histamine - adverse effects; Indomethacin - therapeutic use; Male; Methanol - pharmacology; Phytotherapy; Plant Extracts - adverse effects; Pylorus; Rats; Rats, Wistar; Rubiaceae; Stomach Ulcer - chemically induced; Stomach Ulcer - drug therapy; Stomach Ulcer - pathology; Ulcer - pathology; gastric ulcers; ulcer healing; gastroprotective; Nauclea pobeguinii
• ISSN: 1553-3840
• DOI: 10.1515/JCIM-2023-0024

In this study, we determined the gastroprotective and ulcer-healing effects of extracts (aqueous and methanolic) of
stem-back.
Gastroprotective and healing activity were evaluated following a HCl/ethanol and an indomethacin-induced acute ulcers models; acetic acid, pylorus-ligature, pylorus ligature/histamine and pylorus ligature/acetylcholine-induced chronic ulcers models.
It emerges from this study that, at 100, 200 and 400 mg/kg, the extracts significantly reduced the various ulceration parameters. Compared to negative control male rats, the aqueous (100 mg/kg) and methanolic (400 mg/kg) extracts of
inhibited the ulcers induced by HCl/ethanol by 80.76 % and 100 % respectively, as well as ulcers induced by indomethacin by 88.28 % and 93.47 % respectively. Animals that received 200 mg/kg of both extracts showed a significant reduction in the levels of monocytes, lymphocytes, nitric oxide, MDA and a significant increase in the activities of SOD and catalase. Histological analysis showed repaired mucous epithelium at all doses of both extracts. Aqueous and methanol extracts inhibited ulceration indices by 89.33 % and 88.53 % for pylorus ligature, 83.81 % and 61.07 % for pylorus ligature/acetylcholine and 87.29 % and 99.63 % for pylorus ligature/histamine respectively. Both extracts protected the stomach lining with percentages inhibition of 79.49 % and 81.73 %, respectively in the ethanol test. The extracts induced a significant increase in mucus mass (p<0.001).
The aqueous and methanol extracts of
healed ulcers thanks to their anti-inflammatory, anti-oxidant, anti-secretory and cytoprotective properties.