Molecular and serology methods in the diagnosis of COVID-19: An overview

Coronavirus disease-19 (COVID-19) has become a pandemic, being a global health concern since December 2019 when the first cases were reported. Severe acute respiratory syndrome coronavirus 2, the COVID-19 causal agent, is a β-coronavirus that has on its surface the spike protein, which helps in its...

Full description

Saved in:

Bibliographic Details
Published in:	World journal of methodology Vol. 12; no. 3; pp. 83 - 91
Main Authors:	Luz, Marcel Silva, da Silva Júnior, RT, Santos de Santana, GA, Rodrigues, Gabriela Santos, Crivellaro, Henrique de Lima, Calmon, Mariana Santos, dos Santos, CFSM, Silva, Luis Guilherme de Oliveira, Ferreira, Qesya Rodrigues, Mota, Guilherme Rabelo, Heim, Heloísa, Silva, Filipe Antônio França da, de Brito, BB, de Melo, FF
Format:	Journal Article
Language:	English
Published:	Baishideng Publishing Group Inc 20-05-2022
Subjects:	Minireviews
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Abstract	Coronavirus disease-19 (COVID-19) has become a pandemic, being a global health concern since December 2019 when the first cases were reported. Severe acute respiratory syndrome coronavirus 2, the COVID-19 causal agent, is a β-coronavirus that has on its surface the spike protein, which helps in its virulence and pathogenicity towards the host. Thus, effective and applicable diagnostic methods to this disease come as an important tool for the management of the patients. The use of the molecular technique PCR, which allows the detection of the viral RNA through nasopharyngeal swabs, is considered the gold standard test for the diagnosis of COVID-19. Moreover, serological methods, such as enzyme-linked immunosorbent assays and rapid tests, are able to detect severe acute respiratory syndrome coronavirus 2-specific immunoglobulin A, immunoglobulin M, and immunoglobulin G in positive patients, being important alternative techniques for the diagnostic establishment and epidemiological surveillance. On the other hand, reverse transcription loop-mediated isothermal amplification also proved to be a useful diagnostic method for the infection, mainly because it does not require a sophisticated laboratory apparatus and has similar specificity and sensitivity to PCR. Complementarily, imaging exams provide findings of typical pneumonia, such as the ground-glass opacity radiological pattern on chest computed tomography scanning, which along with laboratory tests assist in the diagnosis of COVID-19.
AbstractList	Coronavirus disease-19 (COVID-19) has become a pandemic, being a global health concern since December 2019 when the first cases were reported. Severe acute respiratory syndrome coronavirus 2, the COVID-19 causal agent, is a β-coronavirus that has on its surface the spike protein, which helps in its virulence and pathogenicity towards the host. Thus, effective and applicable diagnostic methods to this disease come as an important tool for the management of the patients. The use of the molecular technique PCR, which allows the detection of the viral RNA through nasopharyngeal swabs, is considered the gold standard test for the diagnosis of COVID-19. Moreover, serological methods, such as enzyme-linked immunosorbent assays and rapid tests, are able to detect severe acute respiratory syndrome coronavirus 2-specific immunoglobulin A, immunoglobulin M, and immunoglobulin G in positive patients, being important alternative techniques for the diagnostic establishment and epidemiological surveillance. On the other hand, reverse transcription loop-mediated isothermal amplification also proved to be a useful diagnostic method for the infection, mainly because it does not require a sophisticated laboratory apparatus and has similar specificity and sensitivity to PCR. Complementarily, imaging exams provide findings of typical pneumonia, such as the ground-glass opacity radiological pattern on chest computed tomography scanning, which along with laboratory tests assist in the diagnosis of COVID-19.
Author	de Brito, BB Crivellaro, Henrique de Lima de Melo, FF Calmon, Mariana Santos Heim, Heloísa Ferreira, Qesya Rodrigues Luz, Marcel Silva Silva, Filipe Antônio França da Rodrigues, Gabriela Santos Santos de Santana, GA Silva, Luis Guilherme de Oliveira dos Santos, CFSM Mota, Guilherme Rabelo da Silva Júnior, RT
Author_xml	– sequence: 1 givenname: Marcel Silva surname: Luz fullname: Luz, Marcel Silva – sequence: 2 givenname: RT surname: da Silva Júnior fullname: da Silva Júnior, RT – sequence: 3 givenname: GA surname: Santos de Santana fullname: Santos de Santana, GA – sequence: 4 givenname: Gabriela Santos surname: Rodrigues fullname: Rodrigues, Gabriela Santos – sequence: 5 givenname: Henrique de Lima surname: Crivellaro fullname: Crivellaro, Henrique de Lima – sequence: 6 givenname: Mariana Santos surname: Calmon fullname: Calmon, Mariana Santos – sequence: 7 givenname: CFSM surname: dos Santos fullname: dos Santos, CFSM – sequence: 8 givenname: Luis Guilherme de Oliveira surname: Silva fullname: Silva, Luis Guilherme de Oliveira – sequence: 9 givenname: Qesya Rodrigues surname: Ferreira fullname: Ferreira, Qesya Rodrigues – sequence: 10 givenname: Guilherme Rabelo surname: Mota fullname: Mota, Guilherme Rabelo – sequence: 11 givenname: Heloísa surname: Heim fullname: Heim, Heloísa – sequence: 12 givenname: Filipe Antônio França da surname: Silva fullname: Silva, Filipe Antônio França da – sequence: 13 givenname: BB surname: de Brito fullname: de Brito, BB – sequence: 14 givenname: FF surname: de Melo fullname: de Melo, FF
BookMark	eNpVkU1PAjEQhhuDEUSO3nv0stiPbbvrwYTgByQYLuq1Kd1ZqNlttQWM_941EKNzmUnmzfMennPU88EDQpeUjIWU7PrzrR3vKRs7Pi74CRqwbjIiC9b7c_fRKKU3QghlQilSnKE-F4pRlqsBmj2FBuyuMREbX-EEMTRh_YVb2G5ClbDzeLsBXDmz9iG5hEONp8vX-V1Gyxs88TjsIe4dfF6g09o0CUbHPUQvD_fP01m2WD7Op5NFZqkUPKslSFLSkltaM84rWRABK0boiipLJC_B5JZwW8oKlBA8rwypuSjLXKqiqIEP0e2B-75btVBZ8NtoGv0eXWvilw7G6f8f7zZ6Hfa6pEJJJjvA1REQw8cO0la3LlloGuMh7JJmXZHKqRK0i2aHqI0hpQj1bw0l-keA7gToToB2XBecfwPBznj2
Cites_doi	10.1186/s12985-020-01435-6 10.1080/14737159.2020.1757437 10.1016/S2468-1253(20)30083-2 10.1148/radiol.2020200642 10.1016/j.blre.2020.100745 10.3201/eid2607.200841 10.1016/j.dsx.2020.04.011 10.1002/14651858.CD013652 10.1038/nprot.2008.57 10.1016/j.drudis.2020.10.018 10.1016/S0140-6736(20)30154-9 10.2214/AJR.20.23034 10.1002/jmv.25721 10.1016/j.clim.2020.108427 10.1016/j.cca.2020.03.022 10.1002/phar.2395 10.1016/j.ijid.2020.08.029 10.1007/s00330-020-07018-x 10.1016/j.jcv.2020.104412 10.1038/s41591-020-0965-6 10.1016/j.jcv.2020.104511 10.1016/j.acra.2020.04.033 10.1128/JCM.00977-20 10.1515/cclm-2020-0188 10.1080/22221751.2020.1756698 10.1111/1751-7915.13586 10.3390/ijerph17072323 10.1016/j.crad.2020.07.025 10.1126/scitranslmed.abc7075 10.1056/NEJMoa2001017 10.1056/NEJMoa2001191 10.1016/j.jcv.2020.104500 10.1002/jmv.25786 10.1016/j.jpha.2020.02.010 10.1002/jmv.26349 10.1016/j.jacr.2020.03.006 10.1016/j.cmi.2020.11.002 10.1016/j.jmii.2020.03.021 10.1093/nar/28.12.e63 10.1128/jcm.00461-20 10.1111/jth.14768 10.1186/s12879-020-05484-8 10.2214/AJR.20.23391 10.1016/S2468-1253(20)30057-1 10.1016/j.jcv.2020.104468 10.1515/cclm-2020-0369 10.1016/j.nutres.2021.05.008 10.1136/bmj.m2516 10.1016/j.ijid.2020.05.098 10.1016/j.cca.2020.06.045 10.1001/jamanetworkopen.2021.0369 10.3390/ijms21082826 10.1016/j.jcv.2020.104455 10.3390/v11010041 10.21037/qims-20-564 10.1093/ajcp/aqaa112 10.1016/j.cca.2020.08.019 10.1016/j.procbio.2020.08.016 10.1016/S0140-6736(20)30211-7 10.1016/j.bios.2020.112752 10.1155/2020/1023506 10.1080/22221751.2020.1772678 10.1093/infdis/jiaa243 10.3390/biom10060954 10.1016/S0140-6736(20)30183-5 10.1371/journal.pone.0052486 10.1016/j.ijid.2020.09.1451 10.1111/all.14289 10.2144/000114253 10.1093/clinchem/hvaa120 10.1002/jmv.25783 10.1186/s12985-020-01452-5 10.1590/S1678-9946202062044 10.1128/jcm.00599-20 10.1016/j.jcv.2020.104413 10.1016/j.mam.2005.12.007 10.1093/cid/ciaa272 10.1016/j.cmi.2020.04.001 10.1016/j.lfs.2020.118207
ContentType	Journal Article
Copyright	The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved. 2022
Copyright_xml	– notice: The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved. 2022
DBID	AAYXX CITATION 7X8 5PM
DOI	10.5662/wjm.v12.i3.83
DatabaseName	CrossRef MEDLINE - Academic PubMed Central (Full Participant titles)
DatabaseTitle	CrossRef MEDLINE - Academic
DatabaseTitleList
DeliveryMethod	fulltext_linktorsrc
DocumentTitleAlternate	Luz MS et al. Methods in the diagnosis of COVID-19
EISSN	2222-0682
EndPage	91
ExternalDocumentID	10_5662_wjm_v12_i3_83
GroupedDBID	5VR 8WL AAYXX ALMA_UNASSIGNED_HOLDINGS CCEZO CHBEP CIEJG CITATION FA0 HYE RPM 7X8 5PM
ID	FETCH-LOGICAL-c1653-f6e609193c1f233d6805eb201b17c0639ea4c03c96de75534da0f359946788fe3
IEDL.DBID	RPM
ISSN	2222-0682
IngestDate	Fri Sep 01 02:37:44 EDT 2023 Thu Apr 11 20:32:57 EDT 2024 Fri Aug 23 02:33:23 EDT 2024
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	false
IsScholarly	true
Issue	3
Language	English
License	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c1653-f6e609193c1f233d6805eb201b17c0639ea4c03c96de75534da0f359946788fe3
Notes	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 Author contributions: Luz MS contributed to the conceptualization, methodology, validation, investigation, and writing – original draft; da Silva Júnior RT contributed to the validation, visualization, formal analysis, writing – reviewing and editing; Santos de Santana GA, Rodrigues GS, Crivellaro HL, Calmon MS, dos Santos CFSM, Silva LGO, Ferreira QR, Mota GR, and Heim H contributed to the methodology, writing, visualization, investigation, and formal analysis; Silva FAF contributed to the methodology, visualization, investigation, and formal analysis; de Brito BB contributed to the methodology; de Melo FF contributed to the conceptualization, methodology, investigation, writing – original draft, and supervision. Corresponding author: Fabrício Freire de Melo, MSc, PhD, Postdoc, Professor, Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Rua Hormindo Barros, 58, Quadra 17, Lote 58 - Candeias, Vitória da Conquista - BA, 45029-094, Vitória da Conquista 45029-094, Bahia, Brazil. freiremelo@yahoo.com.br
OpenAccessLink	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157626/
PMID	35721247
PQID	2678741751
PQPubID	23479
PageCount	9
ParticipantIDs	pubmedcentral_primary_oai_pubmedcentral_nih_gov_9157626 proquest_miscellaneous_2678741751 crossref_primary_10_5662_wjm_v12_i3_83
PublicationCentury	2000
PublicationDate	2022-5-20 20220520
PublicationDateYYYYMMDD	2022-05-20
PublicationDate_xml	– month: 05 year: 2022 text: 2022-5-20 day: 20
PublicationDecade	2020
PublicationTitle	World journal of methodology
PublicationYear	2022
Publisher	Baishideng Publishing Group Inc
Publisher_xml	– name: Baishideng Publishing Group Inc
References	B20 B64 B21 B65 B22 B66 B23 B67 B24 B68 B25 B69 B26 B27 B28 B29 B70 B71 B72 B73 B30 B74 B31 B75 B32 B76 B33 B77 B34 B78 B35 B79 B36 B37 B38 B39 B1 B2 B3 B4 B5 B6 B7 B8 B9 B80 B81 B40 B41 B42 B43 B44 B45 B46 B47 B48 B49 B50 B51 B52 B53 B10 B54 B11 B55 B12 B56 B13 B57 B14 B58 B15 B59 B16 B17 B18 B19 B60 B61 B62 B63
References_xml	– ident: B37 doi: 10.1186/s12985-020-01435-6 – ident: B10 doi: 10.1080/14737159.2020.1757437 – ident: B17 doi: 10.1016/S2468-1253(20)30083-2 – ident: B28 doi: 10.1148/radiol.2020200642 – ident: B72 doi: 10.1016/j.blre.2020.100745 – ident: B49 doi: 10.3201/eid2607.200841 – ident: B43 doi: 10.1016/j.dsx.2020.04.011 – ident: B45 doi: 10.1002/14651858.CD013652 – ident: B31 doi: 10.1038/nprot.2008.57 – ident: B79 doi: 10.1016/j.drudis.2020.10.018 – ident: B14 doi: 10.1016/S0140-6736(20)30154-9 – ident: B58 doi: 10.2214/AJR.20.23034 – ident: B22 doi: 10.1002/jmv.25721 – ident: B3 doi: 10.1016/j.clim.2020.108427 – ident: B74 doi: 10.1016/j.cca.2020.03.022 – ident: B77 doi: 10.1002/phar.2395 – ident: B53 doi: 10.1016/j.ijid.2020.08.029 – ident: B62 doi: 10.1007/s00330-020-07018-x – ident: B20 doi: 10.1016/j.jcv.2020.104412 – ident: B41 doi: 10.1038/s41591-020-0965-6 – ident: B42 doi: 10.1016/j.jcv.2020.104511 – ident: B59 doi: 10.1016/j.acra.2020.04.033 – ident: B55 doi: 10.1128/JCM.00977-20 – ident: B75 doi: 10.1515/cclm-2020-0188 – ident: B36 doi: 10.1080/22221751.2020.1756698 – ident: B34 doi: 10.1111/1751-7915.13586 – ident: B6 doi: 10.3390/ijerph17072323 – ident: B61 doi: 10.1016/j.crad.2020.07.025 – ident: B2 – ident: B27 doi: 10.1126/scitranslmed.abc7075 – ident: B1 doi: 10.1056/NEJMoa2001017 – ident: B11 doi: 10.1056/NEJMoa2001191 – ident: B56 doi: 10.1016/j.jcv.2020.104500 – ident: B21 doi: 10.1002/jmv.25786 – ident: B18 doi: 10.1016/j.jpha.2020.02.010 – ident: B12 doi: 10.1002/jmv.26349 – ident: B65 doi: 10.1016/j.jacr.2020.03.006 – ident: B38 doi: 10.1016/j.cmi.2020.11.002 – ident: B16 doi: 10.1016/j.jmii.2020.03.021 – ident: B29 doi: 10.1093/nar/28.12.e63 – ident: B23 doi: 10.1128/jcm.00461-20 – ident: B73 doi: 10.1111/jth.14768 – ident: B26 doi: 10.1186/s12879-020-05484-8 – ident: B64 doi: 10.2214/AJR.20.23391 – ident: B71 doi: 10.1016/S2468-1253(20)30057-1 – ident: B48 doi: 10.1016/j.jcv.2020.104468 – ident: B68 doi: 10.1515/cclm-2020-0369 – ident: B80 doi: 10.1016/j.nutres.2021.05.008 – ident: B46 doi: 10.1136/bmj.m2516 – ident: B54 doi: 10.1016/j.ijid.2020.05.098 – ident: B39 doi: 10.1016/j.cca.2020.06.045 – ident: B81 doi: 10.1001/jamanetworkopen.2021.0369 – ident: B33 doi: 10.3390/ijms21082826 – ident: B52 doi: 10.1016/j.jcv.2020.104455 – ident: B4 doi: 10.3390/v11010041 – ident: B63 doi: 10.21037/qims-20-564 – ident: B5 – ident: B47 doi: 10.1093/ajcp/aqaa112 – ident: B67 doi: 10.1016/j.cca.2020.08.019 – ident: B57 doi: 10.1016/j.procbio.2020.08.016 – ident: B7 doi: 10.1016/S0140-6736(20)30211-7 – ident: B50 doi: 10.1016/j.bios.2020.112752 – ident: B60 doi: 10.1155/2020/1023506 – ident: B19 doi: 10.1080/22221751.2020.1772678 – ident: B24 doi: 10.1093/infdis/jiaa243 – ident: B78 doi: 10.3390/biom10060954 – ident: B66 doi: 10.1016/S0140-6736(20)30183-5 – ident: B32 doi: 10.1371/journal.pone.0052486 – ident: B44 doi: 10.1016/j.ijid.2020.09.1451 – ident: B76 doi: 10.1111/all.14289 – ident: B30 doi: 10.2144/000114253 – ident: B40 doi: 10.1093/clinchem/hvaa120 – ident: B70 doi: 10.1002/jmv.25783 – ident: B51 doi: 10.1186/s12985-020-01452-5 – ident: B9 doi: 10.1590/S1678-9946202062044 – ident: B13 doi: 10.1128/jcm.00599-20 – ident: B25 doi: 10.1016/j.jcv.2020.104413 – ident: B15 doi: 10.1016/j.mam.2005.12.007 – ident: B69 doi: 10.1093/cid/ciaa272 – ident: B35 doi: 10.1016/j.cmi.2020.04.001 – ident: B8 doi: 10.1016/j.lfs.2020.118207
SSID	ssj0001257708
Score	2.23526
SecondaryResourceType	review_article
Snippet	Coronavirus disease-19 (COVID-19) has become a pandemic, being a global health concern since December 2019 when the first cases were reported. Severe acute...
SourceID	pubmedcentral proquest crossref
SourceType	Open Access Repository Aggregation Database
StartPage	83
SubjectTerms	Minireviews
Title	Molecular and serology methods in the diagnosis of COVID-19: An overview
URI	https://search.proquest.com/docview/2678741751 https://pubmed.ncbi.nlm.nih.gov/PMC9157626
Volume	12
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
link	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3JTsMwELUoJy4IBIiyyUiIW1I7jh2bW1VA5VBAYhG3KF4igqhbUQq_zzhpoL1yTZRFzyPPe_Z4HkJnXBpWWkuiwqVZlNKURppJHkFsSA3XlaltOocP2e2LvLwKbXJ4examLto3uor9-zj21WtdWzkdm15bJ9a7Hw0UBZaciF4HdYAbLkn0ZmGFZxmRTT9NICtJ7_ttHH_RJK5YLINvDuMge5JgqLKciv745Wp15FK6ud5CmwueiPvN_2yjNed30HDUmtniwlsM0VMviuPGBnqGK4-Bz2HblM9VMzwp8eDu-eYyouoC9z0O9ZphL2AXPV1fPQ6G0cIKITJUcBaVwgnI7IoZWiaMWSEJB01MqKaZCSzDFakhzChhXcY5S21BSsaVgnlQytKxPbTuJ97tIyxl4YgiQpvUpYCiBs4AklTzVGgltO2i8xaVfNp0vMhBKQQkc0ASVHWSVyyXrItOW8xyiMmw0VB4N5nP8gQ-Ckwl47SLshUwf98Yulqv3oHBrrtbLwb34N9PHqKNJJxRIBymgCO0_vkxd8eoM7PzkzpSfgDsCcS2
link.rule.ids	230,315,729,782,786,887,27933,27934,53800,53802
linkProvider	National Library of Medicine
linkToHtml	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3PT9swFLZGd4DLYAJEx2CehHZLasexY3OrWlDRKJs0QNyi-EdEptWtKGX_Ps9JA-2111hxkvde7O-zP7-H0BmXhpXWkqhwaRalNKWRZpJHEBtSw3Vl6jKdoz_ZzYMcXoQ0Obw9C1OL9o2uYv9vEvvqsdZWziam1-rEer_HA0UBJSeit4U-wv9KyApJb5ZWeJYR2WTUBLiS9P7_ncQvNIkrFstQOYdxID5JKKmyOhm9I8x1feTKhHO5u-Gr7qFPS4SJ-03zZ_TB-X00GrdlcHHhLYa4q5fTcVNAeo4rjwEJYtsI76o5npZ48Ov-ahhRdY77HgelZ9hFOEB3lxe3g1G0LKIQGSo4i0rhBGACxQwtE8askIQDmyZU08wEfOKK1BBmlLAu45yltiAl40rBCCpl6dgh6vipd0cIS1k4oojQJnUpfJQGtAFkVvNUaCW07aIfrTXzWZMrIweOETyQgweAjyd5xXLJuuh7a-scojlsURTeTRfzPIGHAsbJOO2ibM0Jbz2GfNjrLWD7Oi_20tZfNr7zG9oe3Y6v8-urm5_HaCcJJx0Ih4HkK-o8Py3cCdqa28VpHW2v6ZvaQg
linkToPdf	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1bb9MwGLXYkCZeGIghCgyMhHhL4kvs2HubelEraKm0gXiL4ku0IOpWlMLf3-ekLe0rvOaez1_sc-yT7yD0XijLa-dIUvm8SHKa08RwJRLIDWVgu7atTef4pph9U4NhLJOzt_pqRfvWNGn4sUhDc9dqK1cLm-10Ytl82tcUUDKT2crV2Ql6CN8sYQdEvZteEUVBVFdVEyALy_58X6S_KUsbnqronsMFkB8WbVUOB6S_KPNYI3kw6IzO_-Nxn6DHW6SJr7tDnqIHPjxD4-nODhdXwWHIv3ZaHXdG0mvcBAyIELtOgNes8bLG_c9fJ4OE6it8HXBUfMbVhAv0ZTS87Y-TrZlCYqkUPKmll4ANNLe0Zpw7qYgAVk2ooYWNOMVXuSXcaul8IQTPXUVqLrSGnlSp2vPn6DQsg3-BsFKVJ5pIY3Ofw4sZQB1Aao3IpdHSuB76sItouepqZpTANWIrlNAKwMtZ2fBS8R56t4t3CVkdlyqq4JebdcngpoB1CkF7qDhqiP0VY13s4z0Q_7Y-9jbeL__5zLfobD4YlZ8ms4-v0CMWf3ggAvqT1-j018-Nv0Qna7d50ybcPeEB3MI
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Molecular+and+serology+methods+in+the+diagnosis+of+COVID-19%3A+An+overview&rft.jtitle=World+journal+of+methodology&rft.au=Luz%2C+Marcel+Silva&rft.au=da+Silva+J%C3%BAnior%2C+Ronaldo+Teixeira&rft.au=Santos+de+Santana%2C+Gabriella+Almeida&rft.au=Rodrigues%2C+Gabriela+Santos&rft.date=2022-05-20&rft.issn=2222-0682&rft.eissn=2222-0682&rft.volume=12&rft.issue=3&rft.spage=83&rft.epage=91&rft_id=info:doi/10.5662%2Fwjm.v12.i3.83&rft.externalDBID=NO_FULL_TEXT
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2222-0682&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2222-0682&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2222-0682&client=summon