Transcriptional Responses of Lacticaseibacillus rhamnosus to TNFα, IL-6, IL-8, and IL-10 Cytokines

• Published in: Biology (Basel, Switzerland) Vol. 13; no. 11; p. 931
• Main Authors: Klimina, Ksenia M., Dyachkova, Marina S., Veselovsky, Vladimir A., Zakharevich, Natalia V., Strokach, Aleksandra A., Selezneva, Oksana V., Shitikov, Egor A., Bespiatykh, Dmitry A., Yunes, Roman A., Poluektova, Elena U., Odorskaya, Maya V., Ostroukhova, Polina S., Bruskin, Sergey A., Danilenko, Valeriy N., Olekhnovich, Evgenii I.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 15-11-2024; MDPI
• Subjects: Bacteria; Cellular stress response; Cytokines; Energy metabolism; Gene expression; Genes; Genomes; Immune system; Inflammatory diseases; Influence; Interleukin 10; Interleukin 6; Interleukin 8; Intestinal microflora; Invoices; Lacticaseibacillus rhamnosus; Microbiota; Microorganisms; Phylogenetics; Probiotics; Tumor necrosis factor-α; Mumbai India; United States > US; Germany; Russia; India
• ISSN: 2079-7737; 2079-7737
• DOI: 10.3390/biology13110931

The interaction between gut microbiota and the host immune system is a complex and understudied field, with cytokines like TNFα, IL-6, IL-8, and IL-10 playing pivotal roles. Commensal bacteria, including lactobacilli, respond to these cytokines through adaptive mechanisms that support their survival and function within the gut. While the influence of cytokines on pathogenic bacteria is well documented, their impact on commensal bacteria, particularly lactobacilli, remains underexplored. This study investigates the transcriptional responses of Lacticaseibacillus rhamnosus strains K32 and R19-3 to various cytokines using next-generation RNA sequencing (RNA-seq). Our findings reveal that cytokines, especially IL-8 and IL-10, significantly alter the L. rhamnosus transcriptome, affecting genes involved in carbohydrate metabolism, stress response, and transcriptional regulation. Notably, IL-8 and IL-10 induce a significant downregulation of genes related to the phosphotransferase system, suggesting a reduction in metabolic activity in response to inflammatory signals. This study unveils a previously unexplored aspect of L. rhamnosus adaptation, highlighting its intricate response to cytokine signals. By modulating gene expression, L. rhamnosus may mitigate the adverse effects of inflammation and promote gut health. These insights could inform the development of targeted probiotic therapies for inflammatory bowel disease (IBD) and other conditions with altered cytokine levels. Our results suggest that co-evolution between a host and gut microbiota enables bacteria to respond to specific cytokines through gene expression changes, revealing a unique and underexplored facet of the interaction between commensal bacteria and the host organism.