Polymicrobial consortia in the pathogenesis of biofilm vaginosis visualized by FISH. Historic review outlining the basic principles of the polymicrobial infection theory

• Published in: Microbes and infection p. 105403
• Main Authors: Swidsinski, Alexander, Amann, Rudolf, Guschin, Alexander, Swidsinski, Sonja, Loening-Baucke, Vera, Mendling, Werner, Sobel, Jack D., Lamont, Ronald F., Vaneechoutte, Mario, Baptista, Pedro Vieira, Bradshaw, Catriona S., Kogan, Igor Yu, Savicheva, Аlevtina M., Mitrokhin, Oleg V., Swidsinski, Nadezhda W., Sukhikh, Gennadiy T., Priputnevich, Tatjana V., Apolikhina, Inna A., Dörffel, Yvonne
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 08-08-2024
• Subjects: 16/23 ribosomal fluorescence in situ hybridization; Bacterial vaginosis; FISH; Infection; Infectious theory; Koch postulates; Pathogenic polymicrobial consortia; Infection; Bacterial vaginosis; 16/23 ribosomal fluorescence in situ hybridization; Koch postulates; Infectious theory; FISH; Pathogenic polymicrobial consortia; bacterial vaginosis; infectious theory; pathogenic polymicrobial consortia
• ISSN: 1286-4579; 1769-714X; 1769-714X
• DOI: 10.1016/j.micinf.2024.105403

The manuscript disputes the exclusive mono-infectious way of thinking, which presumes that for every infection only one pathogen is responsible and sufficient, when infectious vectors, close contact and reduced immunity meet. In situations involving heavily colonized anatomical sites such an approach often ends in insoluble contradictions. Upon critical reflection and evaluation of 20 years research on spatial organization of vaginal microbiota it is apparent, that in some situations, pathogens may act and operate in permanent, structurally organized consortia, whereas its individual components may be innocuous and innocent, failing to express any pathogenic effect. In these cases, consortia are the true pathogens responsible for many infectious conditions, which usually remain unrecognized as long as improperly diagnosed.
The structure of such consortia can be unraveled using ribosomal fluorescence in situ hybridization (FISH). FISH methodology, that not only offers an ex vivo opportunity to recognize bacterial species, but provides unique physical insight into their specific role in the pathogenesis of polymicrobial infections. Ribosomal FISH technique applied to both, women with bacterial vaginosis (BV) and their male partners, has added significantly to our understanding of the pathogenesis of this condition and contributed to appreciating the mechanisms of polymicrobial, community-based infection, potentially leading to therapeutic advances.