Influence of photobiomodulation and radiofrequency on the healing of pressure lesions in mice

• Published in: Lasers in medical science Vol. 39; no. 1; p. 280
• Main Authors: Vieceli, Aline Santo, Silveira, Paulo Cesar Lock, Zaccaron, Rubya Pereira, Fonseca, Marisa de Cássia Registro, Aguiar-Junior, Aderbal Silva, Neves, Lais Mara Siqueira das, Kuriki, Heloyse Uliam, Barbosa, Rafael Inacio, Marcolino, Alexandre Marcio
• Format: Journal Article
• Language: English
• Published: London Springer London 15-11-2024; Springer Nature B.V
• Subjects: Collagen; Dentistry; Fibroblasts; Healing; Infrared analysis; Infrared imaging; Injuries; Injury analysis; Laboratory animals; Lasers; Lesions; Magnets; Medical research; Medicine; Medicine & Public Health; Optical Devices; Optics; Original Article; Photonics; Pressure ulcers; Quantum Optics; Radio frequency; Rehabilitation; Science; Skin; Thermography; Tumor necrosis factor-α; Wound healing; Skin; Pressure ulcers; Wound healing; Low level laser therapy
• ISSN: 1435-604X; 0268-8921; 1435-604X
• DOI: 10.1007/s10103-024-04226-5

The objective of this study was to ascertain the impact of photobiomodulation and radiofrequency on the healing of pressure injuries in mice. A total of 70 animals were randomly assigned to seven experimental groups. A pressure injury was induced in the dorsal region of the mice by the application of two magnets. The photobiomodulation treatment was administered at a dosage of 3.6 J per session. In the radiofrequency group, the treatment time was four minutes and the power was 22 watts. The analyses included the lesion area, infrared thermography, and the collection of material for cytokine, histological, and histochemical analyses following euthanasia. In the macroscopic analyses, the 660 nm photobiomodulation group demonstrated superior outcomes in comparison to the control group. With regard to the microscopic analyses, the greatest difference between the groups was observed when TNF-α was evaluated in the photobiomodulation group. It can be observed that the groups irradiated by electrophysical means (i.e., a combination of radiofrequency with PBM 830 nm–660 nm) exhibited a positive influence on the repair process, with the greatest impact observed in the group irradiated by a combination of radiofrequency and 660 nm photobiomodulation.