Investigation of The Efficacy of Sericin in Experimental Knee Osteoarthritis Model in Rats through the TGF-Beta/Smad Pathway

Investigation of The Efficacy of Sericin in Experimental Knee Osteoarthritis Model in Rats through the TGF-Beta/Smad Pathway

OBJECTIVE: This study investigates the therapeutic efficacy of sericin in rats with knee osteoarthritis (KOA) induced with monosodium iodoacetate (MIA), focusing on evaluating its effectiveness through the TGF-β/Smad pathway. MATERIALS AND METHODS: The KOA model was established by injecting MIA into...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary Sciences and Practices Vol. 19; no. 2; pp. 85 - 93
Main Authors: Gundogdu, Koksal, Kılıç Erkek, Özgen, Gündoğdu, Gülşah, Sayın, Dilek, Abban Mete, Gülçin
Format: Journal Article
Language:English
Published: Atatürk University 30-08-2024
Subjects:
diz osteoartrit
knee osteoarthritis
sericin
serisin
tgf-β1/smad pathway
tgf-β1/smad yolağı
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract OBJECTIVE: This study investigates the therapeutic efficacy of sericin in rats with knee osteoarthritis (KOA) induced with monosodium iodoacetate (MIA), focusing on evaluating its effectiveness through the TGF-β/Smad pathway. MATERIALS AND METHODS: The KOA model was established by injecting MIA into the knee joint and the rats were randomly divided into three groups:group 1 (control), group 2 (KOA control), and group 3 (KOA+sericin). Sericin was administered intratendinously to rats on days 1,7,14, and 21 (50 µL,0.8 g/kg/mL). After 21 days, the rats were sacrificed, and serum samples were analyzed using the ELISA method to measure TGF-β1, Smad2, and CTGF levels. Additionally, knee joint samples underwent histopathological evaluations with hematoxylin-eosin staining and immunohistochemical assessment using TGF-β1 and Smad2/3 antibodies. RESULTS: Serum TGF-β1 and CTGF levels were significantly increased in group 2 vs. group 1 (P
AbstractList OBJECTIVE: This study investigates the therapeutic efficacy of sericin in rats with knee osteoarthritis (KOA) induced with monosodium iodoacetate (MIA), focusing on evaluating its effectiveness through the TGF-β/Smad pathway. MATERIALS AND METHODS: The KOA model was established by injecting MIA into the knee joint and the rats were randomly divided into three groups:group 1 (control), group 2 (KOA control), and group 3 (KOA+sericin). Sericin was administered intratendinously to rats on days 1,7,14, and 21 (50 µL,0.8 g/kg/mL). After 21 days, the rats were sacrificed, and serum samples were analyzed using the ELISA method to measure TGF-β1, Smad2, and CTGF levels. Additionally, knee joint samples underwent histopathological evaluations with hematoxylin-eosin staining and immunohistochemical assessment using TGF-β1 and Smad2/3 antibodies. RESULTS: Serum TGF-β1 and CTGF levels were significantly increased in group 2 vs. group 1 (P
Author Gündoğdu, Gülşah
Kılıç Erkek, Özgen
Abban Mete, Gülçin
Sayın, Dilek
Gundogdu, Koksal
Author_xml – sequence: 1
  givenname: Koksal
  orcidid: 0000-0001-6820-5625
  surname: Gundogdu
  fullname: Gundogdu, Koksal
– sequence: 2
  givenname: Özgen
  orcidid: 0000-0001-8037-099X
  surname: Kılıç Erkek
  fullname: Kılıç Erkek, Özgen
– sequence: 3
  givenname: Gülşah
  orcidid: 0000-0002-9924-5176
  surname: Gündoğdu
  fullname: Gündoğdu, Gülşah
– sequence: 4
  givenname: Dilek
  orcidid: 0000-0003-4022-874X
  surname: Sayın
  fullname: Sayın, Dilek
– sequence: 5
  givenname: Gülçin
  orcidid: 0000-0001-6794-3685
  surname: Abban Mete
  fullname: Abban Mete, Gülçin
BookMark eNpNkVtLAzEQhYMoqNVnX_MH1ua6yT6qVC0qiq3Py2x20kbqRjbxUvDHu7UiwsDMOQwfHM4h2e1ih4SccHbKDavU-B1zcuGUK65VZXfIgbBCFLJkdvffvU-OU3pmjIlKSKvEAfmadu-YclhADrGj0dP5EunE--DArTd6hn1woaPDTD5fB_GCXYYVvekQ6X3KGKHPyz7kkOhdbHG1-XyEnOjgxrfFcthI51eXxTlmGM9eoKUPkJcfsD4iex5WCY9_94g8XU7mF9fF7f3V9OLstnBcGVtob3grtbZKaW7LRrbAG_Tees_EkMKJxpRaO49OW900urFGOVMylLwynMsRmW65bYTn-nWIAP26jhDqHyP2i3rIENwKa9MoxayVDlCqUrJKWgFlha0twYNpB9Z4y3J9TKlH_8fjrP7pot52Uf92Ib8BOYt_yg
Cites_doi 10.2147/JIR.S419186
10.1007/s00441-011-1194-6
10.3390/ijms21103473
10.1016/j.sjbs.2023.103872
10.1007/s00776-009-1401-4
10.1186/1556-276X-6-510
10.1136/ard.2006.061994
10.1126/scitranslmed.3004890
10.1016/j.cytogfr.2011.08.001
10.23736/S0031-0808.19.03822-9
10.3390/gels8090543
10.1007/s00296-015-3290-5
10.5483/BMBRep.2008.41.3.236
10.1007/s00223-014-9917-9
10.1126/scisignal.adf8299
10.1080/09168451.2014.930317
10.1007/s10067-021-05676-w
10.1016/j.biomaterials.2018.02.016
10.1016/j.cca.2020.08.011
10.1186/s40659-018-0204-5
10.1177/2040622312462734
10.1002/jbio.201900204
10.1016/S0142-9612(03)00158-3
10.1016/j.joca.2015.07.018
10.1111/and.13042
10.1002/ctm2.1358
10.3390/ijms21207597
10.1002/1529-0131(200009)43:9<2091::AID-ANR21>3.0.CO;2-C
10.1007/s10067-023-06767-6
10.1016/j.jcpa.2013.12.001
10.1097/BOR.0b013e328349c2b1
10.1016/j.eclinm.2020.100587
10.1016/j.jconrel.2023.12.025
10.1016/j.carbpol.2020.116787
10.1053/joca.1999.0267
10.1177/1759720X211006959
10.1021/acsbiomaterials.8b01521
10.1053/joca.1998.0129
ContentType Journal Article
DBID AAYXX
CITATION
DOA
DOI 10.17094/vetsci.1415498
DatabaseName CrossRef
Directory of Open Access Journals
DatabaseTitle CrossRef
DatabaseTitleList CrossRef
Database_xml – sequence: 1
  dbid: DOA
  name: Directory of Open Access Journals
  url: http://www.doaj.org/
  sourceTypes: Open Website
DeliveryMethod fulltext_linktorsrc
EISSN 2822-3608
EndPage 93
ExternalDocumentID oai_doaj_org_article_7b440883cae346309382a69ed86afa7d
10_17094_vetsci_1415498
GroupedDBID AAYXX
ALMA_UNASSIGNED_HOLDINGS
CITATION
GROUPED_DOAJ
ID FETCH-LOGICAL-c1478-5f71d3558445186b3da1beff8ff02842c2b7655cfec585bb5b874c760e3197113
IEDL.DBID DOA
ISSN 2822-3608
IngestDate Tue Oct 22 15:17:03 EDT 2024
Fri Nov 22 02:16:50 EST 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed false
IsScholarly true
Issue 2
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c1478-5f71d3558445186b3da1beff8ff02842c2b7655cfec585bb5b874c760e3197113
ORCID 0000-0001-6820-5625
0000-0001-8037-099X
0000-0003-4022-874X
0000-0002-9924-5176
0000-0001-6794-3685
OpenAccessLink https://doaj.org/article/7b440883cae346309382a69ed86afa7d
PageCount 9
ParticipantIDs doaj_primary_oai_doaj_org_article_7b440883cae346309382a69ed86afa7d
crossref_primary_10_17094_vetsci_1415498
PublicationCentury 2000
PublicationDate 2024-08-30
PublicationDateYYYYMMDD 2024-08-30
PublicationDate_xml – month: 08
  year: 2024
  text: 2024-08-30
  day: 30
PublicationDecade 2020
PublicationTitle Veterinary Sciences and Practices
PublicationYear 2024
Publisher Atatürk University
Publisher_xml – name: Atatürk University
References ref13
ref35
ref12
ref34
ref15
ref37
ref14
ref36
ref31
ref30
ref11
ref33
ref10
ref32
ref2
ref1
ref17
ref39
ref16
ref38
ref19
ref18
ref24
ref23
ref26
ref25
ref20
ref22
ref21
ref28
ref27
ref29
ref8
ref7
ref9
ref4
ref3
ref6
ref5
References_xml – ident: ref5
  doi: 10.2147/JIR.S419186
– ident: ref32
  doi: 10.1007/s00441-011-1194-6
– ident: ref27
  doi: 10.3390/ijms21103473
– ident: ref21
  doi: 10.1016/j.sjbs.2023.103872
– ident: ref14
  doi: 10.1007/s00776-009-1401-4
– ident: ref18
  doi: 10.1186/1556-276X-6-510
– ident: ref13
  doi: 10.1136/ard.2006.061994
– ident: ref16
  doi: 10.1126/scitranslmed.3004890
– ident: ref10
  doi: 10.1016/j.cytogfr.2011.08.001
– ident: ref15
  doi: 10.23736/S0031-0808.19.03822-9
– ident: ref25
  doi: 10.3390/gels8090543
– ident: ref3
  doi: 10.1007/s00296-015-3290-5
– ident: ref22
  doi: 10.5483/BMBRep.2008.41.3.236
– ident: ref4
  doi: 10.1007/s00223-014-9917-9
– ident: ref12
  doi: 10.1126/scisignal.adf8299
– ident: ref7
  doi: 10.1080/09168451.2014.930317
– ident: ref8
  doi: 10.1007/s10067-021-05676-w
– ident: ref23
  doi: 10.1016/j.biomaterials.2018.02.016
– ident: ref30
  doi: 10.1016/j.cca.2020.08.011
– ident: ref38
– ident: ref17
  doi: 10.1186/s40659-018-0204-5
– ident: ref1
  doi: 10.1177/2040622312462734
– ident: ref24
  doi: 10.1002/jbio.201900204
– ident: ref19
  doi: 10.1016/S0142-9612(03)00158-3
– ident: ref37
  doi: 10.1016/j.joca.2015.07.018
– ident: ref26
  doi: 10.1111/and.13042
– ident: ref9
  doi: 10.1002/ctm2.1358
– ident: ref31
  doi: 10.3390/ijms21207597
– ident: ref36
  doi: 10.1002/1529-0131(200009)43:9<2091::AID-ANR21>3.0.CO;2-C
– ident: ref39
  doi: 10.1007/s10067-023-06767-6
– ident: ref11
  doi: 10.1016/j.jcpa.2013.12.001
– ident: ref6
  doi: 10.1097/BOR.0b013e328349c2b1
– ident: ref2
  doi: 10.1016/j.eclinm.2020.100587
– ident: ref28
  doi: 10.1016/j.jconrel.2023.12.025
– ident: ref29
  doi: 10.1016/j.carbpol.2020.116787
– ident: ref34
  doi: 10.1053/joca.1999.0267
– ident: ref33
  doi: 10.1177/1759720X211006959
– ident: ref20
  doi: 10.1021/acsbiomaterials.8b01521
– ident: ref35
  doi: 10.1053/joca.1998.0129
SSID ssj0002923842
Score 2.3176517
Snippet OBJECTIVE: This study investigates the therapeutic efficacy of sericin in rats with knee osteoarthritis (KOA) induced with monosodium iodoacetate (MIA),...
SourceID doaj
crossref
SourceType Open Website
Aggregation Database
StartPage 85
SubjectTerms diz osteoartrit
knee osteoarthritis
sericin
serisin
tgf-β1/smad pathway
tgf-β1/smad yolağı
Title Investigation of The Efficacy of Sericin in Experimental Knee Osteoarthritis Model in Rats through the TGF-Beta/Smad Pathway
URI https://doaj.org/article/7b440883cae346309382a69ed86afa7d
Volume 19
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrZ1LS8NAEMcX7cmLKCrWF3vw4CU0r33kaDW1IKjYCt7CPlHQVGyqFPzwziSxxJMXIRASlpCdSWb-swm_IeQ0ZSrhkbSBYBkLUi_CAFQRFCuZ4SJzWuoaXzyeiJtHeZkjJmfV6gv_CWvwwI3hBkJjT2SZGOWSlON3OxkrnjkrufJK2Dr6hrxTTGEMjkG3yDRuWT4CapjBh6sgqUBkQCiZ_JWGOrT-Oq2MtshmqwfpeXMf22TNlTvkq0O_mJV05il4k-ZIe1Bmice4qGWeSwpb3mH00-vSOXoLnpvBzJ5qYBHFdmcvOPJeVXPaNuaBvaPTq1EwdJUaTF6VpXcgBj_Vcpc8jPLpxTho2yQEJkqhBmReRBYp6cgak1wnVkXaeS-9B_GQxibWgjNmvDNQG2jNtBSpETx08PqJKEr2SK-clW6fUJPZNDJWWp-BkjNMaZZYb40LjbTCyT45-7Fa8dbQMAqsItDARWPgojVwnwzRqqthiLGuT4Bzi9a5xV_OPfiPixySjRiUSL0QHB6RXvW-cMdkfW4XJ_VD8w22d8ew
link.rule.ids 315,782,786,866,2106,27933,27934
linkProvider Directory of Open Access Journals
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Investigation+of+The+Efficacy+of+Sericin+in+Experimental+Knee+Osteoarthritis+Model+in+Rats+through+the+TGF-Beta%2FSmad+Pathway&rft.jtitle=Veterinary+Sciences+and+Practices&rft.au=Gundogdu%2C+Koksal&rft.au=K%C4%B1l%C4%B1%C3%A7+Erkek%2C+%C3%96zgen&rft.au=G%C3%BCndo%C4%9Fdu%2C+G%C3%BCl%C5%9Fah&rft.au=Say%C4%B1n%2C+Dilek&rft.date=2024-08-30&rft.issn=2822-3608&rft.eissn=2822-3608&rft.volume=19&rft.issue=2&rft.spage=85&rft.epage=93&rft_id=info:doi/10.17094%2Fvetsci.1415498&rft.externalDBID=n%2Fa&rft.externalDocID=10_17094_vetsci_1415498
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2822-3608&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2822-3608&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2822-3608&client=summon