Expression of LMP-1 protein in tumor cells and glandular epithelium of the peritumoral tissue in gastric cancer

• Published in: Arkhiv patologii Vol. 81; no. 6; p. 24
• Main Authors: Danilova, N V, Mikhailov, I A, Oleynikova, N A, Malkov, P G
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2019
• Subjects: Adaptor Proteins, Signal Transducing; Animals; Cytoskeletal Proteins; Epithelium; Humans; LIM Domain Proteins; Lymphatic Metastasis; Mice; Stomach Neoplasms; gastric cancer; immunohistochemistry; LMP-1 protein
• ISSN: 0004-1955
• DOI: 10.17116/patol20198106124

To study the relationship of LMP-1 expression with the clinical and morphological characteristics of gastric cancer and to study LMP-1 expression in the glandular epithelium of the peritumoral tissue.
Samples of gastric cancer surgical material from 67 patients were included in this study. Primary mouse monoclonal antibodies to LMP-1 protein were used. LMP-1 expression was considered as positive if at least in one of the selected 10 fields of view (lens magn. ×40) ratio of the number of cells with LMP-1 expression to the total number of cells exceeded 10%.
Nine cases of LMP-1 positive tumors, 32 cases of LMP-1 negative tumors and 26 cases of LMP-1-negative tumors with LMP-1-expression in the epithelium of the peritumoral tissue were detected. Last group was described for the first time; 4 of these were intestinal type tumors, 19 tumors were 'intermediate' type and 3 tumors were diffuse type, the prevalence of 'intermediate' type tumors was statistically significant.
LMP-1-negative tumors with LMP-1-expression in the epithelium of the peritumoral tissue were identified and described for the first time. This group was predominantly represented by tumors of an 'intermediate' histological type of gastric cancer. EBV antigens may have any direct oncogenic effects, or play the role of the background or the concomitant processes. No significant relationship was found between the LMP-1 expression and depth of invasion (T), lymph nodes metastasis (N), distant metastasis (M), clinical stages. This fact can be explained by the high heterogeneity of molecular and genetic properties of gastric cancer.