Inhibition of bcl-xLexpression by antisense oligonucleotides containing various bridged nucleic acids (BNAs)

• Published in: Nucleic Acids Symposium Series Vol. 51; no. 1; pp. 113 - 114
• Main Authors: Roongjang, Somjing, Takahashi, Kurena, Obika, Satoshi, Imanishi, Takeshi
• Format: Journal Article
• Language: English
• Published: Oxford University Press 01-11-2007
• ISSN: 0261-3166; 1746-8272
• DOI: 10.1093/nass/nrm057

Recently, we described the antisense activity of 2’,4’-BNA oligonucleotides in living cells.1 Here, we examine the antisense effect of 2’,4’-BNA, 2’,4’-COC and 3’-amino-2’,4’-BNA oligonucleotides targeting the bcl-xLgene. The results showed that while S-oligo had a slightly inhibiting effect on bcl-xLexpression and natural DNA had no effect, an antisense oligonucleotide (AON) containing six 2’,4’-COC nucleotides reduced the bcl-xLmRNA level to 64% of the untreated level. Interestingly, several types of AON—those containing nine 2’,4’-BNACOC nucleotides, 2’,4’-BNA and 3’-amino-2’,4’-BNA— completely inhibited expression of the target gene. These data show that, compared with the corresponding natural DNA and S-oligo, BNA-based AONs are efficient inhibitors of bcl-xLexpression.