In vitro sensitivity of Colletotrichum acutatum isolates from strawberry to tebuconazole, prochloraz, fludioxonil and thiophanate-methyl

• Published in: Journal of agricultural sciences (Belgrade, Serbia) Vol. 67; no. 2; pp. 191 - 201
• Main Authors: Milutinovic, Nadja, Vojinovic, Uros, Koprivica, Stasa, Zivanovic, Maja, Vasic, Tanja, Stevic, Milan
• Format: Journal Article
• Language: English
• Published: University of Belgrade - Faculty of Agriculture, Belgrade 2022
• Subjects: anthracnose; fludioxonil; fungicide sensitivity; prochloraz; tebuconazole; thiophanate-methyl
• DOI: 10.2298/JAS2202191M
• EISSN: 2406-0968
• ISSN: 1450-8109

The objective of this study was to determine the Colletotrichum acutatum
isolates sensitivity to several chemical compounds in vitro and to find
their possibilities as a potential control agents for anthracnose disease on
strawberry. C. acutatum J.H. Simmonds, a causing agent of anthracnose, is a
very important pathogen of strawberry, which leads to devastating losses in
its production. The pathogen is effectively controlled by the fungicides.
Thus their application is necessary for achieving high yield and fruit
quality. The sensitivity of 14 isolates of C. acutatum, collected from
commercial strawberry plantations in Serbia, to four fungicides, was
examined by an in vitro sensitivity assay. Based on the results of
morphological, pathogenic and molecular characterization (up to the complex
level), all 14 isolates were determined as C. acutatum. The commercial
formulation of tebuconazole, fludioxonil, prochloraz and thiophanate-methyl
were used for the sensitivity test. The mycelial growth assay method was
used to investigate isolates sensitivity to fungicides. The tested isolates
were very sensitive to prochloraz and fludioxonil, with mean EC50 values of
0.067?0.062 mg L-1 and 0.093?0.043 mg L-1, respectively. Significantly
higher mean EC50 values were observed for tebuconazole (1.473?0.878 mg L-1)
and thiophanate-methyl (1.718?1.592 mg L-1). The toxicity of tested
fungicides in the mycelial growth assay of C. acutatum isolates indicates
the potential implementation of these fungicides in the protection programs
against strawberry anthracnose disease.